2. Recombinant Proteins
  3. Receptor Proteins
  4. S1PR2 Protein, Rat (Cell-Free, His)

S1PR2 Protein, Rat (Cell-Free, His)

Cat. No.: HY-P702426
Handling Instructions

S1PR2 Protein, a receptor for S1P, induces diverse physiological effects in cells and tissues. It also serves as a receptor for FAM19A5, mediating FAM19A5's inhibitory impact on vascular smooth muscle cell proliferation and migration. S1PR2 Protein, Rat (Cell-Free, His) is the recombinant rat-derived S1PR2 protein, expressed by E. coli Cell-free , with C-10*His labeled tag. The total length of S1PR2 Protein, Rat (Cell-Free, His) is 352 a.a., with molecular weight of 41.6 kDa.

For research use only. We do not sell to patients.

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Description

S1PR2 Protein, a receptor for S1P, induces diverse physiological effects in cells and tissues. It also serves as a receptor for FAM19A5, mediating FAM19A5's inhibitory impact on vascular smooth muscle cell proliferation and migration. S1PR2 Protein, Rat (Cell-Free, His) is the recombinant rat-derived S1PR2 protein, expressed by E. coli Cell-free , with C-10*His labeled tag. The total length of S1PR2 Protein, Rat (Cell-Free, His) is 352 a.a., with molecular weight of 41.6 kDa.
Background

The S1PR2 Protein serves as a receptor for the lysosphingolipid sphingosine 1-phosphate (S1P), a bioactive lysophospholipid that induces diverse physiological effects in various cell types and tissues. Additionally, S1PR2 functions as a receptor for the chemokine-like protein FAM19A5, mediating the inhibitory impact of FAM19A5 on vascular smooth muscle cell proliferation and migration.
Species

Rat
Source

E. coli Cell-free
Tag

C-10*His
Accession

P47752 (M1-V352)
Gene ID

29415
Molecular Construction
N-term
S1PR2 (M1-V352)
Accession # P47752
10*His
C-term
Synonyms
Sphingosine 1-phosphate receptor 2; AGR16; Endothelial differentiation G-protein coupled receptor 5; G-protein coupled receptor H218; Sphingosine 1-phosphate receptor Edg-5; S1P receptor Edg-5
AA Sequence

MGGLYSEYLNPEKVQEHYNYTKETLDMQETPSRKVASAFIIILCCAIVVENLLVLIAVARNSKFHSAMYLFLGNLAASDLLAGVAFVANTLLSGPVTLSLTPLQWFAREGSAFITLSASVFSLLAIAIERQVAIAKVKLYGSDKSCRMLMLIGASWLISLILGGLPILGWNCLDHLEACSTVLPLYAKHYVLCVVTIFSVILLAIVALYVRIYFVVRSSHADVAGPQTLALLKTVTIVLGVFIICWLPAFSILLLDSTCPVRACPVLYKAHYFFAFATLNSLLNPVIYTWRSRDLRREVLRPLLCWRQGKGATGRRGGNPGHRLLPLRSSSSLERGLHMPTSPTFLEGNTVV

Molecular Weight

41.6 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

S1PR2 Protein, Rat (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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