SGMS1 Protein, Human (Cell-Free, His)

The SGMS1 protein is a key sphingomyelin synthase located in the Golgi apparatus and promotes sphingomyelin biosynthesis by transferring the phosphocholine moiety. This reversible reaction involves the conversion of phosphatidylcholine and ceramide into sphingomyelin and diacylglycerol. SGMS1 Protein, Human (Cell-Free, His) is the recombinant human-derived SGMS1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SGMS1 Protein, Human (Cell-Free, His) is 413 a.a., with molecular weight of 51.4 kDa.

SGMS1 Protein serves as the primary sphingomyelin synthase at the Golgi apparatus, orchestrating sphingomyelin biosynthesis through reversible phosphocholine transfer. In the forward reaction, it transfers the phosphocholine head group from phosphatidylcholine to ceramide, forming sphingomyelin and diacylglycerol. Conversely, in the reverse reaction, SGMS1 transfers phosphocholine from sphingomyelin to diacylglycerol, generating phosphatidylcholine and ceramide. The directionality depends on Golgi membrane levels of ceramide and diacylglycerol. Notably, SGMS1 does not utilize free phosphorylcholine or CDP-choline as donors. Functionally, it regulates receptor-mediated signal transduction through mitogenic diacylglycerol and proapoptotic ceramide. Furthermore, it influences secretory transport by modulating the DAG pool at the Golgi apparatus, affecting downstream PRKD1 and contributing to membrane raft formation for signal transduction and protein sorting.

Human

E. coli Cell-free

N-10*His

Q86VZ5 (M1-T413)

259230

MKEVVYWSPKKVADWLLENAMPEYCEPLEHFTGQDLINLTQEDFKKPPLCRVSSDNGQRLLDMIETLKMEHHLEAHKNGHANGHLNIGVDIPTPDGSFSIKIKPNGMPNGYRKEMIKIPMPELERSQYPMEWGKTFLAFLYALSCFVLTTVMISVVHERVPPKEVQPPLPDTFFDHFNRVQWAFSICEINGMILVGLWLIQWLLLKYKSIISRRFFCIVGTLYLYRCITMYVTTLPVPGMHFNCSPKLFGDWEAQLRRIMKLIAGGGLSITGSHNMCGDYLYSGHTVMLTLTYLFIKEYSPRRLWWYHWICWLLSVVGIFCILLAHDHYTVDVVVAYYITTRLFWWYHTMANQQVLKEASQMNLLARVWWYRPFQYFEKNVQGIVPRSYHWPFPWPVVHLSRQVKYSRLVNDT

51.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.