SLC25A5 Protein, Human (Sf9, His, MBP, FLAG)

The SLC25A5 protein is an ADP:ATP antiporter on the inner mitochondrial membrane that imports ADP for ATP synthesis and exports ATP for cellular processes. It operates via an alternating access mechanism, cycling between a cytoplasmic open state and a stromal open state. SLC25A5 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC25A5 protein, expressed by Sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag. The total length of SLC25A5 Protein, Human (Sf9, His, MBP, FLAG) is 297 a.a., .

SLC25A5 Protein, an ADP:ATP antiporter, facilitates the import of ADP into the mitochondrial matrix for ATP synthesis and the export of ATP to fuel cellular processes. Operating through the alternating access mechanism, it cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state) on the inner mitochondrial membrane. Beyond its ADP:ATP antiporter activity, SLC25A5 is involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Acting as a proton transporter, it induces mitochondrial uncoupling, disrupting the efficiency of ATP production and promoting mitochondrial thermogenesis. Notably, its proton transporter activity is regulated by ADP:ATP antiporter activity, positioning SLC25A5 as a master regulator of mitochondrial energy output. This delicate balance between ATP production and thermogenesis is further influenced by the requirement of free fatty acids as cofactors for proton transporter activity. SLC25A5 likely mediates mitochondrial uncoupling in tissues lacking UCP1 expression. Additionally, it plays a crucial role in mPTP opening, a non-specific pore contributing to cell death, although its exact role in pore formation remains unclear. Independently of its antiporter activity, SLC25A5 serves as a regulator of mitophagy, promoting mitophagy through interaction with TIMM44, inhibiting TIMM23, and stabilizing PINK1. Moreover, as part of the mitotic spindle-associated MMXD complex, SLC25A5 may contribute to chromosome segregation during mitosis.

Human

Sf9 insect cells

N-MBP;C-Flag;N-8*His

P05141 (T2-T298)

292

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.