STEAP2 Protein, Human (Cell-Free, His, SUMO)

The integral membrane protein, STEAP2, acts as an NADPH-dependent ferric-chelate reductase, facilitating transmembrane electron transfer. It employs NADPH to reduce Fe(3+) chelate, involving sequential electron transfer from NADPH to FAD and then to heme. STEAP2 also demonstrates the ability to reduce Cu(2+) to Cu(1+), showcasing its versatility in transmembrane electron transfer processes. STEAP2 Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived STEAP2 protein, expressed by E. coli Cell-free , with N-10*His, N-SUMO labeled tag. The total length of STEAP2 Protein, Human (Cell-Free, His, SUMO) is 490 a.a., with molecular weight of 74.6 kDa.

The STEAP2 protein, an integral membrane protein, serves as an NADPH-dependent ferric-chelate reductase, employing NADPH from one side of the membrane to reduce a Fe(3+) chelate bound on the opposite side. Operating through sequential transmembrane electron transfer, STEAP2 facilitates the transfer of electrons from NADPH to FAD and then onto heme, ultimately reducing the Fe(3+) chelate. Additionally, STEAP2 exhibits the capability to reduce Cu(2+) to Cu(1+), adding versatility to its role as a mediator of transmembrane electron transfer processes.

Human

E. coli Cell-free

N-10*His;N-SUMO

Q8NFT2 (M1-M490)

261729

MESISMMGSPKSLSETFLPNGINGIKDARKVTVGVIGSGDFAKSLTIRLIRCGYHVVIGSRNPKFASEFFPHVVDVTHHEDALTKTNIIFVAIHREHYTSLWDLRHLLVGKILIDVSNNMRINQYPESNAEYLASLFPDSLIVKGFNVVSAWALQLGPKDASRQVYICSNNIQARQQVIELARQLNFIPIDLGSLSSAREIENLPLRLFTLWRGPVVVAISLATFFFLYSFVRDVIHPYARNQQSDFYKIPIEIVNKTLPIVAITLLSLVYLAGLLAAAYQLYYGTKYRRFPPWLETWLQCRKQLGLLSFFFAMVHVAYSLCLPMRRSERYLFLNMAYQQVHANIENSWNEEEVWRIEMYISFGIMSLGLLSLLAVTSIPSVSNALNWREFSFIQSTLGYVALLISTFHVLIYGWKRAFEEEYYRFYTPPNFVLALVLPSIVILGKIILFLPCISRKLKRIKKGWEKSQFLEEGMGGTIPHVSPERVTVM

74.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.