Structural polyprotein Protein, Ross river virus (Cell-Free, His)

The structural polyprotein directs the assembly of a T=4 symmetric icosahedral capsid that houses 240 copies of the capsid protein in the lipid membrane and is decorated with 80 E1-E2 spike heterodimers. Functionally, it binds viral RNA, enables translation after genome release, and exhibits autocatalytic protease activity. Structural polyprotein Protein, Ross river virus (Cell-Free, His) is the recombinant Virus-derived Structural polyprotein protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Structural polyprotein Protein, Ross river virus (Cell-Free, His) is 438 a.a., with molecular weight of 50.3 kDa.

The Structural polyprotein Protein orchestrates the formation of an icosahedral capsid with T=4 symmetry, comprised of 240 capsid protein copies encased in a lipid membrane, and adorned with 80 spikes composed of E1-E2 heterodimeric trimers. Functionally, the capsid protein binds to the viral RNA genome, enabling its translation following genome release, and possesses protease activity leading to autocatalytic cleavage. Post-cleavage, it associates with ribosomes and rapidly assembles into icosahedral core particles before associating with the spike glycoprotein E2 at the cell membrane, promoting budding and mature virion formation. During infection, new virions attach to target cells, undergo clathrin-mediated endocytosis, and release the nucleocapsid into the cytoplasm through membrane fusion. The capsid protein, potentially triggering uncoating, exhibits a dual role in genomic RNA and ribosome binding. Moreover, it specifically inhibits interleukin-1 receptor-associated kinase 1/IRAK1-dependent signaling during viral entry, serving as a mechanism for evading innate immune detection. Additionally, the protein provides a signal sequence for translocating the precursor of protein E3/E2 to the host endoplasmic reticulum. Furin-cleaved E3, associated with spike glycoprotein E1, ensures pH protection during transport in the secretory pathway, with gradual release into the extracellular space upon virion release from the host cell.

Virus

E. coli Cell-free

N-10*His

P08491 (Y817-R1254)

/

YEHTATIPNVVGFPYKAHIERNGFSPMTLQLEVVETSWEPTLNLEYITCEYKTVVPSPFIKCCGTSECSSKEQPDYQCKVYTGVYPFMWGGAYCFCDSENTQLSEAYVDRSDVCKHDHASAYKAHTASLKATIRISYGTINQTTEAFVNGEHAVNVGGSKFIFGPISTAWSPFDNKIVVYKDDVYNQDFPPYGSGQPGRFGDIQSRTVESKDLYANTALKLSRPSPGVVHVPYTPTPSGFKYWLKEKGSSLNTKAPFGCKIKTNPVRAMDCAVGSIPVSMDIPDSAFTRVVDAPAVTDLSCQVVVCTHSSDFGGVATLSYKTDKPGKCAVHSHSNVATLQEATVDVKEDGKVTVHFSTASASPAFKVSVCDAKTTCTAACEPPKDHIVPYGASHNNQVFPDMSGTAMTWVQRLASGLGGLALIAVVVLVLVTCITMRR

50.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.