TM4SF2/TSPAN7 Protein, Cynomolgus (HEK293, His)

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TM4SF2/TSPAN7 protein is an important member of the tetraspanin family and plays an important role in signal transduction, cell adhesion, and membrane organization. Its research enhances understanding of cell membrane dynamics and provides potential applications in cell biology and cancer research. TM4SF2/TSPAN7 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TM4SF2/TSPAN7 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Cynomolgus
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

TM4SF2/TSPAN7 protein is an important member of the tetraspanin family and plays an important role in signal transduction, cell adhesion, and membrane organization. Its research enhances understanding of cell membrane dynamics and provides potential applications in cell biology and cancer research. TM4SF2/TSPAN7 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TM4SF2/TSPAN7 protein, expressed by HEK293 , with N-His labeled tag.

Background

The TM4SF2/TSPAN7 Protein is a pivotal member of the tetraspanin (TM4SF) family, highlighting its essential role in various cellular processes, including signal transduction, cell adhesion, and membrane organization. As part of this family, TM4SF2/TSPAN7 likely shares conserved structural and functional features with related proteins, emphasizing its involvement in the formation of tetraspanin-enriched microdomains and interactions with other cellular molecules. The classification within the tetraspanin family underscores its specific designation within the broader context of membrane proteins, providing insights into its unique contributions to cell membrane dynamics. The study of TM4SF2/TSPAN7 contributes to our understanding of its role in diverse physiological processes, offering potential applications in cell biology, cancer research, and a deeper comprehension of its broader impact on cellular functions. Further exploration of TM4SF2/TSPAN7's role holds promise for enhancing our knowledge of its contributions to both normal physiology and pathological conditions.

Verified Bioactivity

When Recombinant Cynomolgus TSPAN7 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Anti-TSPAN7 Antibody. The ED50 for this effect is 171.7 ng/mL.

Technical Parameters

  • Species Cynomolgus
  • Source HEK293
  • Tag N-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • His
    • TM4SF2 (R113-M213)
      Accession # Q4R5A3
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    Mxs1; Tm4sf2; Tetraspanin-7; Tspan-7; Cell surface glycoprotein A15; PE31; TALLA homolog; Transmembrane 4 superfamily member 2; CD antigen; CD231; XLID58; CCG-B7; TM4SF2b; Tetraspanin; Tetraspanin Protein; Transmembrane 4 Superfamily 2b; Transmembrane Pro

  • AA Sequence

    RHEIKDTFLRTYTDAMQNYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLDHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNM

  • Predicted Molecular Mass

    11.6 kDa

  • Molecular Weight

    Approximately 20-28 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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