UGT1A8 Protein, Human (Cell-Free, His)

UGT1A8 protein is a key UDP-glucuronosyltransferase (UGT) that plays a vital role in phase II biotransformation by catalyzing the conjugation of lipophilic substrates with glucuronic acid, enhancing water solubility, and promoting excretion. role. UGT1A8 Protein, Human (Cell-Free, His) is the recombinant human-derived UGT1A8 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of UGT1A8 Protein, Human (Cell-Free, His) is 505 a.a., with molecular weight of 59.9 kDa.

UGT1A8, a pivotal member of the UDP-glucuronosyltransferase (UGT) family, takes center stage in phase II biotransformation reactions by catalyzing the conjugation of lipophilic substrates with glucuronic acid, enhancing water solubility and facilitating excretion into urine or bile. This enzymatic prowess is indispensable for the elimination and detoxification of drugs, xenobiotics, and endogenous compounds. UGT1A8 exhibits a broad substrate specificity, extending its catalytic reach to endogenous steroid hormones, including androgens and estrogens, as well as phytoestrogens such as genistein and daidzein, known for their anticancer and cardiovascular properties. Notably, UGT1A8 orchestrates a series of glucuronidation steps to produce dihydrotestosterone (DHT) diglucuronide from DHT. Additionally, this versatile enzyme contributes to the glucuronidation of the AGTR1 angiotensin receptor antagonist caderastan and metabolizes mycophenolate, an immunosuppressive agent. Intriguingly, UGT1A8, while lacking glucuronidation activity, functions as a negative regulator of isoform 1, underscoring its intricate role in cellular processes.

Human

E. coli Cell-free

N-10*His

Q9HAW9 (G26-H530)

54576

GKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLGKSLNCTVKTYSTSYTLEDLDREFMDFADAQWKAQVRSLFSLFLSSSNGFFNLFFSHCRSLFNDRKLVEYLKESSFDAVFLDPFDACGLIVAKYFSLPSVVFARGIACHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLFCQYFSKNALEIASEILQTPVTAYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH

59.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.