ZWINT/ZW10 interactor Protein, Human (GST)

ZWINT, a vital MIS12 complex component, is essential for kinetochore and spindle checkpoint activity. It plays a pivotal role in ZW10 targeting to the kinetochore in prometaphase, forming interactions with ZW10, MIS12, and NDC80 subunit of the NDC80 complex. ZWINT's engagement with KNL1, CETN3, DSN1, and PMF1 underscores its multifaceted role in orchestrating kinetochore complex assembly and functionality during mitosis. ZWINT/ZW10 interactor Protein, Human (GST) is the recombinant human-derived ZWINT/ZW10 interactor protein, expressed by E. coli , with N-GST labeled tag. The total length of ZWINT/ZW10 interactor Protein, Human (GST) is 277 a.a., with molecular weight of ~58.2 kDa.

ZWINT, as a key component of the MIS12 complex, plays a pivotal role in kinetochore formation and spindle checkpoint activity. Its involvement is crucial for the proper targeting of ZW10 to the kinetochore during prometaphase. ZWINT establishes interactions with essential partners within the complex, including ZW10 and MIS12. Furthermore, ZWINT exhibits a specific interaction with the NDC80 subunit of the NDC80 complex during mitosis, contributing to the orchestration of intricate cellular processes associated with spindle dynamics. In addition to these interactions, ZWINT engages with KNL1, CETN3, DSN1, and PMF1, highlighting its multifaceted role in facilitating the assembly and functionality of the kinetochore complex.

Human

E. coli

N-GST

O95229 (1M-277P)

11130  [NCBI]

MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Approximately 58.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.