Cibinetide
Based on 6 publication(s) in Google Scholar
Cibinetide (ARA290) is an erythropoietin (EPO) derivative that acts as a specific agonist of the erythropoietin/CD131 heteroreceptor. ICibinetide can be used in the research of neurological disorders.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 1208243-50-8
- Formula: C51H84N16O21
- Molecular Weight:1257.31
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cibinetide
More
Customer Validation & Images
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IHC
Biological Activity
Cibinetide (ARA290) enhances the proliferation, migration, and resistance to H2O2-induced apoptosis of endothelial colony-forming cells (ECFCs)[1]. Cibinetide (ARA290) is an EPO-analog peptide without hematopoietic side-effects but may have neurotrophic and antidepressant effects[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1208243-50-8
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Appearance Solid
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Molecular Weight 1257.31
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Formula C51H84N16O21
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Color White to off-white
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Synonyms
ARA290
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Sequence
{Glp}-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser
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Sequence Shortening
{Glp}-EQLERALNSS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (6)
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Journal Impact Factor
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Most Recent
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CNS Neurosci Ther
Erythropoietin-derived peptide ARA290 mediates brain tissue protection through the β-common receptor in mice with cerebral ischemic stroke. [Abstract]2024 Mar;30(3):e14676. PMID: 38488446 -
Immunology
EPOR activation attenuates colitis via enhancing LC3B-dependent apoptotic cell clearance. [Abstract]2025 Sep;176(1):33-48. PMID: 37204242 -
Int J Mol Sci
2024 Mar 29;25(7):3854. PMID: 38612664 -
Toxicol In Vitro
An engineered non-erythropoietic erythropoietin-derived peptide, ARA290, attenuates doxorubicin induced genotoxicity and oxidative stress. [Abstract]2020 Aug;66:104864. PMID: 32335150
Cibinetide purchased from MedChemExpress. Usage Cited in: Toxicol In Vitro. 2020 Aug;66:104864. [Abstract]
Representative photomicrographs demonstrate the results of the TUNEL assay. Alterations in apoptotic cells are assessed using the ratio of green fluorescence. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Solvent & Solubility
H2O : 66.67 mg/mL (53.03 mM; adjust pH to 8 with NH3·H2O)
0.1 M NaOH : ≥ 50 mg/mL (39.77 mM)
DMSO : ≥ 34 mg/mL (27.04 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 2.5 mg/mL (1.99 mM); Clear solution; Need ultrasonic and warming and heat to 37°C
Protocol
Diabetic Goto-Kakizaki (GK) rats, originating from Wistar rats, are bred in our department. Normal Wistar (W) rats are used as nondiabetic controls. All animals are about six weeks old and with body weights 100 to 150 g when treatment is initiated. They are kept at 22°C on a reversed 12-h light-dark cycle with free access to food, except when fasted overnight as noted below. Rats are treated over 4 wks with Cibinetide by a once daily subcutaneous (s.c.) injection at a dose of 30 μg/kg body weight or PBS. Blood samples for determination of glucose are taken after a small tail incision and analyzed every week before morning s.c. injection of either Cibinetide or placebo. During the experimental period, body weights are measured weekly.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hache G, et al. ARA290, a Specific Agonist of Erythropoietin/CD131 Heteroreceptor, Improves Circulating Endothelial Progenitors' Angiogenic Potential and Homing Ability. Shock. 2016 Oct;46(4):390-7 [Content Brief]
[2]. Carole Muller, et al. ARA290 Improves Insulin Release and Glucose Tolerance in Type 2 Diabetic Goto-Kakizaki Rats. Mol Med. 2015; 21(1): 969–978 [Content Brief]
[3]. Chen H, et al. Therapeutic effects of nonerythropoietic erythropoietin analog ARA290 in experimental autoimmune encephalomyelitis rat. J Neuroimmunol. 2014 Mar 15;268(1-2):64-70 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / 0.1 M NaOH / H2O | 1 mM | 0.7953 mL | 3.9767 mL | 7.9535 mL | 19.8837 mL |
| 5 mM | 0.1591 mL | 0.7953 mL | 1.5907 mL | 3.9767 mL | |
| 10 mM | 0.0795 mL | 0.3977 mL | 0.7953 mL | 1.9884 mL | |
| 15 mM | 0.0530 mL | 0.2651 mL | 0.5302 mL | 1.3256 mL | |
| 20 mM | 0.0398 mL | 0.1988 mL | 0.3977 mL | 0.9942 mL | |
| 25 mM | 0.0318 mL | 0.1591 mL | 0.3181 mL | 0.7953 mL | |
| 0.1 M NaOH / H2O | 30 mM | 0.0265 mL | 0.1326 mL | 0.2651 mL | 0.6628 mL |
| H2O | 40 mM | 0.0199 mL | 0.0994 mL | 0.1988 mL | 0.4971 mL |
| 50 mM | 0.0159 mL | 0.0795 mL | 0.1591 mL | 0.3977 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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