AZD1208 hydrochloride
Based on 22 publication(s) in Google Scholar
AZD1208 hydrochloride is an orally bioavailable, highly selective PIM kinases inhibitor.
For research use only. We do not sell to patients.
- CAS No.: 1621866-96-3
- Formula: C21H22ClN3O2S
- Molecular Weight:415.94
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
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Publications Citing Use of MedChemExpress (MCE) AZD1208 hydrochloride
More- Science. 2017 Dec 1;358(6367):eaan4368. [Abstract]
- Nat Commun. 2019 Apr 23;10(1):1844. [Abstract]
- Sci Transl Med. 2018 Jul 18;10(450):eaaq1093. [Abstract]
- Adv Sci (Weinh). 2025 Jul 6:e03487. [Abstract]
- Cell Chem Biol. 2024 Feb 15;31(2):326-337.e11. [Abstract]
- Neurosci Bull. 2024 Dec;40(12):1901-1914. [Abstract]
- Biochem Pharmacol. 2026 Jan;243(Pt 2):117536. [Abstract]
- Int Immunopharmacol. 2023 Jan:114:109563. [Abstract]
- mBio. 2025 Oct 8;16(10):e0168025. [Abstract]
- Cell Signal. 2024 Jan:113:110969. [Abstract]
- PLoS Negl Trop Dis. 2026 Jan 20;20(1):e0013915. [Abstract]
- PLoS One. 2025 Nov 12;20(11):e0335501. [Abstract]
- Prostate. 2025 Apr 23. [Abstract]
- eNeuro. 2019 Aug 22;6(4):ENEURO.0003-19.2019. [Abstract]
- Exp Ther Med. 2022 Jan;23(1):81. [Abstract]
- bioRxiv. 2025 Nov 3:2025.10.31.685966. [Abstract]
- bioRxiv. 2024 Nov 6:2024.11.04.621884. [Abstract]
- bioRxiv. 2024 Mar 28.
- Research Square Preprint. 2024 Feb 16.
- The University of Chicago. 2021 Apr.
- Charles University. 2019 Jun.
- Methods Mol Biol. 2018:1711:351-398. [Abstract]
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Biological Activity
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PIM2 |
AZD1208 hydrochloride shows good antiproliferative activity in a megakaryoblastic leukemia cell line, MOLM-16, with GI50 values less than 100 nM[1]. AZD1208 hydrochloride (10 μM) inhibits the growth of Ramos cells, and at 1 μM, strongly inhibits PIM kinases in all cells at 1 μM. AZD1208 hydrochloride induces apoptosis, and PIM2 knockdown is mainly associated with an alteration of the cell cycle[2]. The combination of AZD1208 hydrochloride and AZD2014 rapidly activates AMPKα, a negative regulator of translation machinery through mTORC1/2 signaling in AML cells; profoundly inhibits AKT and 4EBP1 activation; and suppresses polysome formation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1621866-96-3
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Molecular Weight 415.94
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Formula C21H22ClN3O2S
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SMILES
O=C(NC/1=O)SC1=C/C2=C(N3C[C@H](N)CCC3)C(C4=CC=CC=C4)=CC=C2.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (22)
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Journal Impact Factor
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Most Recent
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Science
2017 Dec 1;358(6367):eaan4368. PMID: 29191878 -
Nat Commun
PIM kinases facilitate lentiviral evasion from SAMHD1 restriction via Vpx phosphorylation. [Abstract]2019 Apr 23;10(1):1844. PMID: 31015445
AZD1208 hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Apr 23;10(1):1844. [Abstract]
Primary monocytes were differentiated into macrophages and infected with HIV-2 reporter virus carrying wild-type Vpx or its S13A mutants. A pan-PIM kinase inhibitor AZD1208 (0, 0.1, 0.5 μM) was added 4 h before infection. Fortyeight hours post-infection, cells were harvested and subjected to luciferase assay.
AZD1208 hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Apr 23;10(1):1844. [Abstract]
Primary monocytes were differentiated into macrophages and infected with HIV-2 reporter virus carrying wild-type Vpx or its S13A mutants. A pan-PIM kinase inhibitor AZD1208 (0, 0.1, 0.5 μM) was added 4 h before infection. Fortyeight hours post-infection, cells were harvested and immunoblotting to measure HIV-2 infectivity and SAMHD1 expression.
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Sci Transl Med
PP2A inhibition is a druggable MEK inhibitor resistance mechanism in KRAS-mutant lung cancer cells. [Abstract]2018 Jul 18;10(450):eaaq1093. PMID: 30021885 -
Adv Sci (Weinh)
PIM1 Attenuates Innate Immunity to Foster Coronavirus Replication through Ubiquitin Ligase β-TrCP-Mediated IFNAR1 Degradation. [Abstract]2025 Jul 6:e03487. PMID: 40619619
AZD1208 hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 6:e03487. [Abstract]
Protein level of p-STAT1/2 in HEK293T cells treated with PIM1 inhibitor AZD-1208 (0, 2, 4, 8 μM) for 24h followed by the stimulation with/without poly: IC for 16 h, or IFN for 1h.
AZD1208 hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 6:e03487. [Abstract]
PIM inhibitors AZD-1208 (0-12 μM) pre-treated with RD cells for 2h, followed by the OC43 infection for 72h at the MOI of 0.01. OC43 viral RNA were determined.
AZD1208 hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 6:e03487. [Abstract]
PIM inhibitors AZD-1208 (0-12 μM) pre-treated with RD cells for 2h, followed by the OC43 infection for 72h at the MOI of 0.01. OC43 viral N protein was determined.
AZD1208 hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 6:e03487. [Abstract]
Protein level of -TrCP1 in HEK293T cells treated with the indicated concentration of PIM1 inhibitor AZD-1208 (0-12 μM) for 48h.
AZD1208 hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 6:e03487. [Abstract]
Western blot for IFNAR1 expression in HEK293T or HepG2 cells treated with PIM1 inhibitors AZD-1208 (0, 2, 4, 8 μM) for 48 h.
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Cell Chem Biol
2024 Feb 15;31(2):326-337.e11. PMID: 38016478 -
Neurosci Bull
Melatonin Ameliorates Abnormal Sleep-Wake Behavior via Facilitating Lipid Metabolism in a Zebrafish Model of Parkinson's Disease. [Abstract]2024 Dec;40(12):1901-1914. PMID: 39283564 -
Biochem Pharmacol
Targeting PIM1 ameliorates sepsis-associated acute kidney injury through inhibiting ferroptosis. [Abstract]2026 Jan;243(Pt 2):117536. PMID: 41207569 -
Int Immunopharmacol
2023 Jan:114:109563. PMID: 36513021 -
mBio
PIM1 orchestrates sepsis-associated inflammatory imbalance in CD4+ T cell subsets via cholesterol metabolism. [Abstract]2025 Oct 8;16(10):e0168025. PMID: 40899834 -
Cell Signal
2024 Jan:113:110969. PMID: 37967691 -
PLoS Negl Trop Dis
Unraveling the role of host kinase PIM1 in Toxoplasma gondii infection: Implications for therapies. [Abstract]2026 Jan 20;20(1):e0013915. PMID: 41557744 -
PLoS One
PIM1 induces hypoxia-related fibroblast senescence in a mouse model of stress urinary incontinence. [Abstract]2025 Nov 12;20(11):e0335501. PMID: 41223182 -
Prostate
Valosin-Containing Protein (VCP/p97) Mediates Neuroendocrine Differentiation in Prostate Cancer Cells Through Pim1 Signaling Inducing Autophagy. [Abstract]2025 Apr 23. PMID: 40269472 -
eNeuro
2019 Aug 22;6(4):ENEURO.0003-19.2019. PMID: 31387876 -
Exp Ther Med
2022 Jan;23(1):81. PMID: 34934450 -
bioRxiv
2025 Nov 3:2025.10.31.685966. PMID: 41279778 -
bioRxiv
PAIRWISE: Deep Learning-based Prediction of Effective Personalized Drug Combinations in Cancer. [Abstract]2024 Nov 6:2024.11.04.621884. PMID: 39574568 -
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Methods Mol Biol
2018:1711:351-398. PMID: 29344898
Solvent & Solubility
DMSO : 83.3 mg/mL (200.27 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
MOLM-16 cells, purchased from DSMZ and cultured in RPMI containing 10% fetal bovine serum (FBS) and 1% L-glutamine, are plated at 20,000 cells per well in 96 well plates overnight. Cells are treated for 72 hours with compound (including AZD1208 hydrochloride) or control vehicle (dimethyl sulfoxide) and cell viability is measured after the addition of Cell Titer-Blue for 4 hours at 37˚C and reading of fluorescence on a Tecan Infinite® 200. The GI50 is determined by calculating growth at each dose relative to vehicle treated cells and cell viability at the time of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
References
[1]. Dakin LA, et al. Discovery of novel benzylidene-1,3-thiazolidine-2,4-diones as potent and selective inhibitors of the PIM-1, PIM-2, and PIM-3 protein kinases. Bioorg Med Chem Lett. 2012 Jul 15;22(14):4599-604. [Content Brief]
[2]. Kreuz S, et al. Loss of PIM2 enhances the anti-proliferative effect of the pan-PIM kinase inhibitor AZD1208 in non-Hodgkin lymphomas. Mol Cancer. 2015 Dec 8;14:205. [Content Brief]
[3]. Harada M, et al. The novel combination of dual mTOR inhibitor AZD2014 and pan-PIM inhibitor AZD1208 inhibits growth in acute myeloid leukemia via HSF pathway suppression. Oncotarget. 2015 Nov 10;6(35):37930-47. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4042 mL | 12.0210 mL | 24.0419 mL | 60.1048 mL |
| 5 mM | 0.4808 mL | 2.4042 mL | 4.8084 mL | 12.0210 mL | |
| 10 mM | 0.2404 mL | 1.2021 mL | 2.4042 mL | 6.0105 mL | |
| 15 mM | 0.1603 mL | 0.8014 mL | 1.6028 mL | 4.0070 mL | |
| 20 mM | 0.1202 mL | 0.6010 mL | 1.2021 mL | 3.0052 mL | |
| 25 mM | 0.0962 mL | 0.4808 mL | 0.9617 mL | 2.4042 mL | |
| 30 mM | 0.0801 mL | 0.4007 mL | 0.8014 mL | 2.0035 mL | |
| 40 mM | 0.0601 mL | 0.3005 mL | 0.6010 mL | 1.5026 mL | |
| 50 mM | 0.0481 mL | 0.2404 mL | 0.4808 mL | 1.2021 mL | |
| 60 mM | 0.0401 mL | 0.2003 mL | 0.4007 mL | 1.0017 mL | |
| 80 mM | 0.0301 mL | 0.1503 mL | 0.3005 mL | 0.7513 mL | |
| 100 mM | 0.0240 mL | 0.1202 mL | 0.2404 mL | 0.6010 mL |
AZD1208 hydrochloride Related Classifications
HY-15604A Related Classifications
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