1. Anti-infection
  2. Bacterial

Closthioamide 

Cat. No.: HY-101472
Handling Instructions

Closthioamide is a potent inhibitor of bacterial DNA gyrase and highly active against Ec, MRSA, VRE and Mv), with MICs of 9.00 μM, 0.58 μM, 0.58 μM and 72.03 μM respectively.

For research use only. We do not sell to patients.

Closthioamide Chemical Structure

Closthioamide Chemical Structure

CAS No. : 1227367-59-0

Size Stock
250 mg Get quote
500 mg Get quote

* Please select Quantity before adding items.

Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Closthioamide is a potent inhibitor of bacterial DNA gyrase and highly active against Ec, MRSA, VRE and Mv), with MICs of 9.00 μM, 0.58 μM, 0.58 μM and 72.03 μM respectively.

IC50 & Target

Bacterial DNA gyrase[1].
MIC: 9.00 μM (Ec), 0.58 μM (MRSA), 0.58 μM (VRE), 72.03 μM (Mv)[2].

In Vitro

Closthioamide is a potent inhibitor of bacterial DNA gyrase, isolated from the strictly anaerobic banterium Clostridium cellulolyticum and belongs to a new class of natural products[1]. In a standardized antimicrobial assay, it is found that closthioamide is highly active against a pathogenic, methicillin-resistant Staphylococcus aureus (MRSA) strain with a minimum inhibitory concentration (MIC) of 0.4 μg/mL-1 (0.58 μM). Closthioamide is even active against vancomycin-resistant Enterococcus faecalis (VRE) with the same low MIC value, and is thus significantly more potent against these bacteria than ciprofloxacin, the standard drugused against VRE, with remarkable strain selectivity. Furthermore, in a standardized cytotoxicity assay, closthioamide shows moderate antiproliferative and cytotoxic effects[2].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4388 mL 7.1938 mL 14.3877 mL
5 mM 0.2878 mL 1.4388 mL 2.8775 mL
10 mM 0.1439 mL 0.7194 mL 1.4388 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The MIC is determined for the laboratory strains as follows. The assays are performed in sterile 96-well polystyrene microplates in 200 mL of cation-adjusted Mueller-Hinton (MH) broth. A serial dilution of the compound to test is made in 100 mL of medium and inoculated with 100 mL of fresh cell culture containing ~105 cfu/mL and incubated at 37°C for 20 h. The MIC is defined as the lowest concentration of antibiotic that inhibit visible growth[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

695.04

Formula

C₂₉H₃₈N₆O₂S₆

CAS No.

1227367-59-0

SMILES

S=C(C1=CC=C(O)C=C1)NCCC(NCCC(NCCCNC(CCNC(CCNC(C2=CC=C(O)C=C2)=S)=S)=S)=S)=S

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Closthioamide
Cat. No.:
HY-101472
Quantity:

Closthioamide

Cat. No.: HY-101472