Dansylcadaverine (Synonyms: Monodansyl cadaverine)

Cat. No.: HY-D1027 Purity: 98.62%: FAQs
Data Sheet SDS COA Handling Instructions

Dansylcadaverine (Monodansyl cadaverine) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.

For research use only. We do not sell to patients.

Dansylcadaverine Chemical Structure

CAS No. : 10121-91-2

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10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

5 Publications Citing Use of MCE Dansylcadaverine

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

In Vitro

The inhibitory activity of dansylcadaverine reflects its ability to serve as a substrate for transglutaminases and to block competitively the crosslinking of fibrin molecules^[2].
Dansylcadaverine, a cationic fluorescent probe binds to bacterial lipopolysaccharide and lipid A, and is displaced competitively by other compounds which possess affinity toward endotoxins^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

335.46

Appearance

Solid

Formula

C₁₇H₂₅N₃O₂S

CAS No.

10121-91-2

SMILES

NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (186.31 mM; Need ultrasonic)

H₂O : 1 mg/mL (2.98 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9810 mL	14.9049 mL	29.8098 mL
5 mM	0.5962 mL	2.9810 mL	5.9620 mL
10 mM	0.2981 mL	1.4905 mL	2.9810 mL

*Please refer to the solubility information to select the appropriate solvent.

Purity & Documentation

Purity: 98.62%

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Data Sheet (276 KB) SDS (393 KB)

COA (248 KB) HNMR (229 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

Dyeing Example

References

[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. [Content Brief]

[2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9. [Content Brief]

[3]. Gao L, et al. Autophagy blockade sensitizes human head and neck squamous cell carcinoma towards CYT997 through enhancing excessively high reactive oxygen species-induced apoptosis. J Mol Med (Berl). 2018;96(9):929-938. [Content Brief]

[4]. Cornwell MM, et al. Inhibition of the adhesion of Chinese hamster ovary cells by the naphthylsulfonamides dansylcadaverine and N-(6-aminohexyl)-5-chloro-1-naphthylenesulfonamide (W7). Biochim Biophys Acta. 1983;762(3):414-419. [Content Brief]

[5]. David SA, et al. Analysis of the binding of polymyxin B to endotoxic lipid A and core glycolipid using a fluorescent displacement probe. Biochim Biophys Acta. 1992;1165(2):147-152. [Content Brief]

Kinase Assay ^[1]	To determine the time course of transglutamination, thymosin β₄ (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl₂, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay	MCF7 cells (2.4×10⁴) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. [Content Brief] [2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9. [Content Brief] [3]. Gao L, et al. Autophagy blockade sensitizes human head and neck squamous cell carcinoma towards CYT997 through enhancing excessively high reactive oxygen species-induced apoptosis. J Mol Med (Berl). 2018;96(9):929-938. [Content Brief] [4]. Cornwell MM, et al. Inhibition of the adhesion of Chinese hamster ovary cells by the naphthylsulfonamides dansylcadaverine and N-(6-aminohexyl)-5-chloro-1-naphthylenesulfonamide (W7). Biochim Biophys Acta. 1983;762(3):414-419. [Content Brief] [5]. David SA, et al. Analysis of the binding of polymyxin B to endotoxic lipid A and core glycolipid using a fluorescent displacement probe. Biochim Biophys Acta. 1992;1165(2):147-152. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

Dansylcadaverine10121-91-2Monodansyl cadaverineAutophagyautofluorescentprobeautophagicvacuolessubstrateInhibitorinhibitorinhibit

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Dansylcadaverine (Synonyms: Monodansyl cadaverine)

Customer Review

5 Publications Citing Use of MCE Dansylcadaverine

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

Your Recently Viewed Products:

Customer Review

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