Dansylcadaverine
Based on 14 publication(s) in Google Scholar
Dansylcadaverine (Monodansyl cadaverine) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.
For research use only. We do not sell to patients.
- Purity: 98.80%
- CAS No.: 10121-91-2
- Formula: C17H25N3O2S
- Molecular Weight:335.46
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Dansylcadaverine
More- Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]
- J Extracell Vesicles. 2025 Aug;14(8):e70133. [Abstract]
- Microbiome. 2025 Jan 31;13(1):33. [Abstract]
- Int J Biol Sci. 2021 Jul 13;17(11):2970-2983. [Abstract]
- New Phytol. 2026 Jun;250(5):3084-3102. [Abstract]
- Br J Pharmacol. 2025 Aug;182(16):3923-3951. [Abstract]
- Biomed Pharmacother. 2024 Jun:175:116614. [Abstract]
- Cell Prolif. 2024 Apr 16:e13646. [Abstract]
- Int J Pharm. 2022 Nov 25:628:122297. [Abstract]
- Neurochem Res. 2023 May;48(5):1395-1411. [Abstract]
- Biochim Biophys Acta Mol Cell Res. 2022 Mar;1869(3):119173. [Abstract]
- Toxicol Appl Pharmacol. 2020 Dec 15;409:115271. [Abstract]
- bioRxiv. 2025 Sep 16:2025.09.11.674989. [Abstract]
- Res Sq. 2024 Aug 07.
Customer Validation & Images
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IF
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Flow Cytometry
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Histological Imaging/Staining
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WB
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Bio/Physico-chemical Assay
Biological Activity
The inhibitory activity of dansylcadaverine reflects its ability to serve as a substrate for transglutaminases and to block competitively the crosslinking of fibrin molecules[2].
Dansylcadaverine, a cationic fluorescent probe binds to bacterial lipopolysaccharide and lipid A, and is displaced competitively by other compounds which possess affinity toward endotoxins[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 10121-91-2
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Appearance Solid
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Molecular Weight 335.46
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Formula C17H25N3O2S
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Color White to off-white
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SMILES
NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O
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Synonyms
Monodansyl cadaverine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen)
Publications (14)
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Journal Impact Factor
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Most Recent
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Gastroenterology
2025 Dec 10:S0016-5085(25)05907-4. PMID: 41369634
Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]
Endocytosis inhibitor Dansylcadaverine (DC) (80 μM; i.v.; twice a week) inhibited the endocytosis of OG-collagen IV (green) by mesothelial cells.
Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]
Dansylcadaverine (DC) (80 μM; i.v.; twice a week) significantly inhibited the endocytosis effect of collagen by mesothelial cells mediated by CLUtide-3-LRP2 interaction.
Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]
SR and Masson staining in fibrotic livers from Clu-/- mice treated with Dansylcadaverine (DC) (80 μM; i.v.; twice a week) as indicated.
Dansylcadaverine purchased from MedChemExpress. Usage Cited in: Gastroenterology. 2025 Dec 10:S0016-5085(25)05907-4. [Abstract]
Col1A1 and quantification in fibrotic livers from Clu-/- mice treated with Dansylcadaverine (DC) (80 μM; i.v.; twice a week) as indicated.
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J Extracell Vesicles
Syntenin Controls Extracellular Vesicle-Induced Tumour Migration by Regulating the Expression of Adhesion Proteins on Small Extracellular Vesicles. [Abstract]2025 Aug;14(8):e70133. PMID: 40831280
Dansylcadaverine purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70133. [Abstract]
Dansylcadaverine (Da) (180 µM; 24 h) was able to efficiently block vesicle incorporation into 4T1 cells by 50% at a concentration that was non-toxic for the cells.
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Microbiome
A tale for two roles: Root-secreted methyl ferulate inhibits P. nicotianae and enriches the rhizosphere Bacillus against black shank disease in tobacco. [Abstract]2025 Jan 31;13(1):33. PMID: 39891202 -
Int J Biol Sci
MicroRNA-216b targets HK2 to potentiate autophagy and apoptosis of breast cancer cells via the mTOR signaling pathway. [Abstract]2021 Jul 13;17(11):2970-2983. PMID: 34345220 -
New Phytol
Lipid metabolism and autophagy maintain cellular homeostasis under chloroplast dysfunction in Chlamydomonas reinhardtii. [Abstract]2026 Jun;250(5):3084-3102. PMID: 41999165 -
Br J Pharmacol
A mitochondria-targeting and G-quadruplex structure-binding ligand inducing calcium overload and ferroptosis in human cancer cells. [Abstract]2025 Aug;182(16):3923-3951. PMID: 40344208 -
Biomed Pharmacother
Pseudolaric acid B suppresses NSCLC progression through the ROS/AMPK/mTOR/autophagy signalling pathway. [Abstract]2024 Jun:175:116614. PMID: 38670047 -
Cell Prolif
Deletion of Tgm2 suppresses BMP-mediated hepatocyte-to-cholangiocyte metaplasia in ductular reaction. [Abstract]2024 Apr 16:e13646. PMID: 38623945 -
Int J Pharm
ClC-3 silencing mediates lysosomal acidification arrest and autophagy inhibition to sensitize chemo-photothermal therapy. [Abstract]2022 Nov 25:628:122297. PMID: 36261097 -
Neurochem Res
Kaemperfol Protects Dopaminergic Neurons by Promoting mTOR-Mediated Autophagy in Parkinson's Disease Models. [Abstract]2023 May;48(5):1395-1411. PMID: 36469163 -
Biochim Biophys Acta Mol Cell Res
2022 Mar;1869(3):119173. PMID: 34902478 -
Toxicol Appl Pharmacol
Targeting autophagy by natural product Ursolic acid for prevention and treatment of osteoporosis. [Abstract]2020 Dec 15;409:115271. PMID: 33065153 -
bioRxiv
Insulin-Like Growth Factor 1 Receptor Regulates Breast Cancer Cell Adhesion through Beta-1 Integrin. [Abstract]2025 Sep 16:2025.09.11.674989. PMID: 41000977 -
Solvent & Solubility
DMSO : 62.5 mg/mL (186.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
To determine the time course of transglutamination, thymosin β4 (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl2, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MCF7 cells (2.4×104) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. [Content Brief]
[2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9. [Content Brief]
[3]. Gao L, et al. Autophagy blockade sensitizes human head and neck squamous cell carcinoma towards CYT997 through enhancing excessively high reactive oxygen species-induced apoptosis. J Mol Med (Berl). 2018;96(9):929-938. [Content Brief]
[4]. Cornwell MM, et al. Inhibition of the adhesion of Chinese hamster ovary cells by the naphthylsulfonamides dansylcadaverine and N-(6-aminohexyl)-5-chloro-1-naphthylenesulfonamide (W7). Biochim Biophys Acta. 1983;762(3):414-419. [Content Brief]
[5]. David SA, et al. Analysis of the binding of polymyxin B to endotoxic lipid A and core glycolipid using a fluorescent displacement probe. Biochim Biophys Acta. 1992;1165(2):147-152. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.9810 mL | 14.9049 mL | 29.8098 mL | 74.5245 mL |
| 5 mM | 0.5962 mL | 2.9810 mL | 5.9620 mL | 14.9049 mL | |
| 10 mM | 0.2981 mL | 1.4905 mL | 2.9810 mL | 7.4525 mL | |
| 15 mM | 0.1987 mL | 0.9937 mL | 1.9873 mL | 4.9683 mL | |
| 20 mM | 0.1490 mL | 0.7452 mL | 1.4905 mL | 3.7262 mL | |
| 25 mM | 0.1192 mL | 0.5962 mL | 1.1924 mL | 2.9810 mL | |
| 30 mM | 0.0994 mL | 0.4968 mL | 0.9937 mL | 2.4842 mL | |
| 40 mM | 0.0745 mL | 0.3726 mL | 0.7452 mL | 1.8631 mL | |
| 50 mM | 0.0596 mL | 0.2981 mL | 0.5962 mL | 1.4905 mL | |
| 60 mM | 0.0497 mL | 0.2484 mL | 0.4968 mL | 1.2421 mL | |
| 80 mM | 0.0373 mL | 0.1863 mL | 0.3726 mL | 0.9316 mL | |
| 100 mM | 0.0298 mL | 0.1490 mL | 0.2981 mL | 0.7452 mL |
Dansylcadaverine Related Classifications
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