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  2. Dansylcadaverine

Dansylcadaverine (Synonyms: Monodansyl cadaverine)

Cat. No.: HY-D1027 Purity: 98.00%
Handling Instructions

Dansylcadaverine is a fluorescent probe.

For research use only. We do not sell to patients.

Dansylcadaverine Chemical Structure

Dansylcadaverine Chemical Structure

CAS No. : 10121-91-2

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Based on 1 publication(s) in Google Scholar

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Description

Dansylcadaverine is a fluorescent probe.

In Vitro

Detection of Dansylcadaverine fluorescence after SDS-PAGE shows that the amount of actin-thymosin β4 crosslink is very low in the presence of Dansylcadaverine and transglutaminase, while there are large amounts of Dansylcadaverine-labeled thymosin β4 and actin. The amount of cross-link further decreases in the presence of Dansylcadaverine competing for Lys residues of either G-actin or thymosin β4 (100 μM). In the absence of thymosin β4, Dansylcadaverine-labeled actin polymerized to F-actin as revealed by fluorescence detection after native PAGE[1].

Storage

-20°C, protect from light, stored under nitrogen

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (186.31 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9810 mL 14.9049 mL 29.8098 mL
5 mM 0.5962 mL 2.9810 mL 5.9620 mL
10 mM 0.2981 mL 1.4905 mL 2.9810 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

To determine the time course of transglutamination, thymosin β4 (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl2, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay

MCF7 cells (2.4×104) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

335.46

Formula

C₁₇H₂₅N₃O₂S

CAS No.

10121-91-2

SMILES

NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere

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Dansylcadaverine
Cat. No.:
HY-D1027
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Dansylcadaverine

Cat. No.: HY-D1027