2. Anti-infection
  3. HIV
  4. RN-18

RN-18 

Cat. No.: HY-102014 Purity: 98.75%
COA Handling Instructions

RN-18 is a HIV-1 viral infectivity factor (HIV-1 Vif) inhibitor with an IC50 of 6 μM in nonpermissive H9 cells.

For research use only. We do not sell to patients.

RN-18 Chemical Structure

RN-18 Chemical Structure

CAS No. : 431980-38-0

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 131 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 131 In-stock
Solid
5 mg USD 119 In-stock
10 mg USD 198 In-stock
25 mg USD 422 In-stock
50 mg USD 713 In-stock
100 mg USD 1241 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

RN-18 is a HIV-1 viral infectivity factor (HIV-1 Vif) inhibitor with an IC50 of 6 μM in nonpermissive H9 cells.

IC50 & Target

IC50: 6 μM (nonpermissive H9 cell)[1]
In Vitro

RN-18 and RN-19 exhibits potent antiviral activity in the nonpermissive H9 and CEM cells but not in MT4 or CEM-SS cells, confirming that the antiviral activity was Vif specific. RN-18 shows the greater potency (IC50=4.5 μM in CEM cells) and specificity (IC50>100 μM in MT4 cells) among the two compounds[1]. In the presence of the inhibitor, RN-18, reverse transcriptase activity in the nonpermissive H9 and CEM cells decreases substantially and in a dose-dependent manner. RN-18 also exhibits antiviral activity in CEM-SS modified to stably express A3G but does not exhibit antiviral activity in the parental CEM-SS cell line. RN-18 antagonizes Vif function and inhibits HIV-1 replication only in the presence of A3G. RN-18 increases cellular A3G levels in a Vif-dependent manner and increases A3G incorporation into virions without inhibiting general proteasome-mediated protein degradation. RN-18 enhances Vif degradation only in the presence of A3G, reduces viral infectivity by increasing A3G incorporation into virions and enhances cytidine deamination of the viral genome[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

380.42

Appearance

Solid

Formula

C20H16N2O4S
CAS No.
SMILES

O=C(NC1=CC=CC=C1OC)C2=CC=CC=C2SC3=CC=C([N+]([O-])=O)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (262.87 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6287 mL 13.1434 mL 26.2867 mL
5 mM 0.5257 mL 2.6287 mL 5.2573 mL
10 mM 0.2629 mL 1.3143 mL 2.6287 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
References
Cell Assay
[2]

H9 or MT4 cells are treated overnight with 0, 1, 5, 10, 25 or 50 μM RN-18 (all at 0.1% DMSO) and infected with HIV-1. All cells are maintained in the presence of DMSO or RN-18 for 14 d, and viral replication is monitored every 2 d by measuring reverse transcriptase activity in culture supernatants. The average % relative infectivity at day 7 is determined from 3 separate reverse transcriptase assays. Grafit software is used to fit curves and to determine IC50[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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C1   V1   C2   V2

Keywords:

RN-18431980-38-0RN18RN 18HIVHuman immunodeficiency virusInhibitorinhibitorinhibit

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