1. Dye Reagents

Rhod-2 AM 

Cat. No.: HY-D0989
Handling Instructions

Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm).

For research use only. We do not sell to patients.

Rhod-2 AM Chemical Structure

Rhod-2 AM Chemical Structure

CAS No. : 129787-64-0

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Customer Review

    Rhod-2 AM purchased from MCE. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948.

    Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa2O3 treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.
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    Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm).

    In Vitro

    A high concentration of Ca2+ in mitochondria is illustrated by punctate labeling in cells, which is consistent with the location of Ca2+ in the mitochondria following fluorescence staining with Rhod-2 AM (Rhod2-AM) at 6 h p.i.. Inhibition of mitochondrial Ca2+ uptake by ruthenium red (RR) or 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) in IMR5 cells infected with poliovirus (PV) is also illustrated following fluorescence staining with Rhod-2 AM at 6 h p.i.[1].

    Cell Assay

    For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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    Please store the product under the recommended conditions in the Certificate of Analysis.


    Room temperature in continental US; may vary elsewhere

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    Product Name:
    Rhod-2 AM
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    Rhod-2 AM

    Cat. No.: HY-D0989