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Rhod-2 AM 

Cat. No.: HY-D0989 Purity: ≥96.0%
COA Handling Instructions

Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm.

For research use only. We do not sell to patients.

Rhod-2 AM Chemical Structure

Rhod-2 AM Chemical Structure

CAS No. : 145037-81-6

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50 μg USD 60 In-stock
1 mg USD 580 In-stock
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Customer Review

Based on 11 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948.  [Abstract]

    Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa2O3 treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.
    • Biological Activity

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    Description

    Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm[1].

    In Vitro

    1.Preparation of Rhod-2 AM working solution
    1.1Preparation of the stock solution
    Dissolve Rhod-2 AM in DMSO to obtain 5 mM of stock solution.
    1.2Preparation of Rhod-2 AM working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of working solution.
    Note: Please adjust the concentration of Rhod-2 AM working solution according to the actual situation.
    2.Cell staining (6-well plate)
    2.1Suspension cells
    a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
    d.Wash twice with PBS, 5 minutes each time.
    e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a.Culture adherent cells on sterile coverslips.
    b.Remove the coverslip from the medium and aspirate excess medium.
    c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
    d.Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    1123.94

    Formula

    C52H59BrN4O19

    CAS No.
    Appearance

    Oil

    Color

    Green to dark green

    Emission (Em)

    578

    Excitation (Ex)

    549

    SMILES

    CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC5=CC(C)=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4)=C2C=C1)C.[Br-]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Purity & Documentation

    Purity: ≥97.0%

    Dyeing Example
    References
    Cell Assay
    [1]

    For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Rhod-2 AM Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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