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  4. Rhod-2 AM

Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm.

For research use only. We do not sell to patients.

CAS No. : 145037-81-6

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Customer Review

Based on 33 publication(s) in Google Scholar

Other Forms of Rhod-2 AM:

Rhod-2 AM Related Antibodies

Top Publications Citing Use of Products

33 Publications Citing Use of MCE Rhod-2 AM

IF
Cell Imaging/Staining

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030.  [Abstract]

    CLSM images showing membrane integrity and calcium levels of mitochondria in 4T1 cells treated with various nanoreactors. Rhod‐2 AM was dissolved in DMSO containing 0.02% Pluronic F127 and diluted using PBS to a final concentration of 5 µM. 4T1 cells were seeded in confocal dishes and cultured for 24 h. Nanoreactors (200 µg/mL) were added to the culture for an additional 24 h. Following three washes with PBS, the cells were incubated with the probe for 30 min, followed by imaging with CLSM.

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821.  [Abstract]

    To detect the level of Ca2+ in mitochondria, C. albicans cells were stained with Rhod-2 AM (5 μM, 30 min) at 37 °C. The images were acquired utilizing a confocal microscope. The excitation and emission wavelengths for the fluorescent probes used in this study are as follows: ER-Tracker Red (587 nm/615 nm), Rhod-2AM (549 nm/578 nm).

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 21;11(47):eadz4126.  [Abstract]

    RAW 264.7 cells were harvested and stained with a mitochondrial Ca2+ indicator (Rhod-2 AM) at room temperature for 30 min.

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 May 7:S2090-1232(24)00175-9.  [Abstract]

    To detect intracellular Ca2+, cells were treated with 1 μM of Rhod-2 AM fluorescent probes and subsequently washed. The intracellular Ca2+ levels were quantified.

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948.  [Abstract]

    Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa2O3 treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm[1].

    In Vitro

    Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
    1. Preparation of Rhod-2 AM Solution 1.1 Preparation of the Solution Dissolve Rhod-2 AM in DMSO to obtain a 5 mM stock solution.
    1.2 Preparation of Rhod-2 AM Solution Dilute the stock solution with serum-free cell culture medium or PBS to obtain a working solution of 5-10 μM.
    Note: Please adjust the concentration of the Rhod-2 AM working solution according to the actual situation.
    2. Cell Staining (6-well plate) 2.1 Suspended Cells a. Centrifuge at 4°C for 3-5 minutes at 1000g. Discard the supernatant. Wash twice with PBS, each time for 5 minutes. The cell density is 1×106 cells/mL.
    b. Add 1 mL of the working solution, and then incubate at room temperature for 5 to 30 minutes.
    c. Centrifuge at 4°C for 3-4 minutes at 400g, then discard the supernatant.
    d. Wash twice with PBS, for 5 minutes each time.
    e. Resuspend the cells in serum-free cell culture medium or PBS. Observe the cells using a fluorescence microscope or flow cytometry.
    2.2 Wall-adhering cells a. Cultivate adherent cells on a sterile cover slip.
    b. Remove the cover glass from the culture medium and aspirate the excess culture medium.
    c. Add 100 μL of the working solution, gently shake to mix well, ensuring that the cells are fully covered. Incubate at room temperature for 5 to 30 minutes.
    d. Wash twice with the culture medium, for 5 minutes each time. Observe under a fluorescence microscope or flow cytometer.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Rhod-2 AM Related Antibodies
    Molecular Weight

    1123.94

    Formula

    C52H59BrN4O19
    CAS No.
    Appearance

    Solid

    Color

    Dark green to black

    Emission (Em)

    578
    Excitation (Ex)

    549
    SMILES

    CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC5=CC(C)=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4)=C2C=C1)C.[Br-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 37.5 mg/mL (33.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.8897 mL 4.4486 mL 8.8973 mL
    5 mM 0.1779 mL 0.8897 mL 1.7795 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: ≥97.0%

    SDS (252 KB)

    COA (259 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Cell Assay
    [1]

    For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.8897 mL 4.4486 mL 8.8973 mL 22.2432 mL
    5 mM 0.1779 mL 0.8897 mL 1.7795 mL 4.4486 mL
    10 mM 0.0890 mL 0.4449 mL 0.8897 mL 2.2243 mL
    15 mM 0.0593 mL 0.2966 mL 0.5932 mL 1.4829 mL
    20 mM 0.0445 mL 0.2224 mL 0.4449 mL 1.1122 mL
    25 mM 0.0356 mL 0.1779 mL 0.3559 mL 0.8897 mL
    30 mM 0.0297 mL 0.1483 mL 0.2966 mL 0.7414 mL
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    Rhod-2 AM Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Rhod-2 AM145037-81-6Fluorescent DyeInhibitorinhibitorinhibit

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