2. Academic Validation
  3. Identification of the TPO1 gene in yeast, and its human orthologue TETRAN, which cause resistance to NSAIDs

Identification of the TPO1 gene in yeast, and its human orthologue TETRAN, which cause resistance to NSAIDs

  • FEBS Lett. 2007 Apr 3;581(7):1457-63. doi: 10.1016/j.febslet.2007.03.001.
Shinji Mima 1,  Hironori Ushijima,  Hyun-Jung Hwang,  Shinji Tsutsumi,  Masaki Makise,  Yoshihiro Yamaguchi,  Tomofusa Tsuchiya,  Hiroshi Mizushima,  Tohru Mizushima
Affiliations

Affiliation

  • 1 Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan.
PMID: 17362938 DOI: 10.1016/j.febslet.2007.03.001
Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs), such as indomethacin, have serious gastrointestinal side effects. Since their direct cytotoxicity was suggested to be involved in this side effect, we here tried to identify NSAID-resistant genes. We screened for Saccharomyces cerevisiae genes whose overexpression causes indomethacin resistance and identified the TPO1 gene, which encodes a major facilitator superfamily transporter. Its overexpression or deletion made yeast cells resistant or sensitive, respectively, to some NSAIDs. A BLAST search identified the possible human orthologue of Tpo1p, Tetracycline transporter-like protein (TETRAN), whose overexpression in cultured human cells caused resistance to some NSAIDs, suggesting that TETRAN is an efflux pump for some NSAIDs.

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