2. Academic Validation
  3. Reversible Ponceau staining as a loading control alternative to actin in Western blots

Reversible Ponceau staining as a loading control alternative to actin in Western blots

  • Anal Biochem. 2010 Jun 15;401(2):318-20. doi: 10.1016/j.ab.2010.02.036.
Isabel Romero-Calvo 1,  Borja Ocón,  Patricia Martínez-Moya,  María Dolores Suárez,  Antonio Zarzuelo,  Olga Martínez-Augustin,  Fermín Sánchez de Medina
Affiliations

Affiliation

  • 1 Department of Biochemistry, School of Pharmacy, University of Granada, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Campus de Cartuja s/n, 18071 Granada, Spain.
PMID: 20206115 DOI: 10.1016/j.ab.2010.02.036
Abstract

It is becoming standard practice to measure a housekeeping gene, typically actin, in Western blots, as it is the rule in RNA blots. We have applied reversible Ponceau staining to check equal loading of gels and measured actin in parallel under different conditions. Our results show that densitometric analysis is comparable with both techniques. Therefore, routine quantitation of Ponceau staining before antibody probing is validated as an alternative to actin blotting.

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