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  4. Ponceau S

Ponceau S  (Synonyms: Acid Red 112)

Cat. No.: HY-12489 Purity: ≥98.0%
COA Handling Instructions

Molarity Calculator

Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red.

For research use only. We do not sell to patients.

Ponceau S Chemical Structure

Ponceau S Chemical Structure

CAS No. : 6226-79-5

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Based on 1 publication(s) in Google Scholar

Ponceau S Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red[1][2][3].

In Vitro

Ponceau S 的浓度 ( 0.001-2 (w/v); 5-10 分钟) 、酸浓度和酸类型 (乙酸、三氯乙酸/磺基水杨酸)，不影响蛋白质检测的灵敏度[1]。

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ponceau S Related Antibodies
Molecular Weight

760.57

Formula

C22H12N4Na4O13S4
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (131.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 35.71 mg/mL (46.95 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3148 mL 6.5740 mL 13.1480 mL
5 mM 0.2630 mL 1.3148 mL 2.6296 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 8.33 mg/mL (10.95 mM); Clear solution; Need ultrasonic and warming

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (254 KB) HNMR (530 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.3148 mL 6.5740 mL 13.1480 mL 32.8701 mL
5 mM 0.2630 mL 1.3148 mL 2.6296 mL 6.5740 mL
10 mM 0.1315 mL 0.6574 mL 1.3148 mL 3.2870 mL
15 mM 0.0877 mL 0.4383 mL 0.8765 mL 2.1913 mL
20 mM 0.0657 mL 0.3287 mL 0.6574 mL 1.6435 mL
25 mM 0.0526 mL 0.2630 mL 0.5259 mL 1.3148 mL
30 mM 0.0438 mL 0.2191 mL 0.4383 mL 1.0957 mL
40 mM 0.0329 mL 0.1644 mL 0.3287 mL 0.8218 mL
DMSO 50 mM 0.0263 mL 0.1315 mL 0.2630 mL 0.6574 mL
60 mM 0.0219 mL 0.1096 mL 0.2191 mL 0.5478 mL
80 mM 0.0164 mL 0.0822 mL 0.1644 mL 0.4109 mL
100 mM 0.0131 mL 0.0657 mL 0.1315 mL 0.3287 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ponceau S Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ponceau S6226-79-5Acid Red 112Acid Red112Acid Red-112Fluorescent DyestainWesternblottingacidicantibody-antigenproteinsredInhibitorinhibitorinhibit

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