Ponceau S
Based on 1 Customer Validation
Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 6226-79-5
- Formula: C22H12N4Na4O13S4
- Molecular Weight:760.57
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Concentration of Ponceau S (0.001-2 (w/v); 5-10 min), acid concentration and acid type (acetic acid, trichloroacetic acid/sulfosalicylic acid) doesn’t affect the sensitivity of protein detection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6226-79-5
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Appearance Solid
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Molecular Weight 760.57
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Formula C22H12N4Na4O13S4
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Color Brown to black
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SMILES
O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O
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Synonyms
Acid Red 112
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 100 mg/mL (131.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 35.71 mg/mL (46.95 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53. [Content Brief]
[2]. Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020(3): 098459. [Content Brief]
[3]. Romero-Calvo I, et al. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem. 2010 Jun 15;401(2):318-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.3148 mL | 6.5740 mL | 13.1480 mL | 32.8701 mL |
| 5 mM | 0.2630 mL | 1.3148 mL | 2.6296 mL | 6.5740 mL | |
| 10 mM | 0.1315 mL | 0.6574 mL | 1.3148 mL | 3.2870 mL | |
| 15 mM | 0.0877 mL | 0.4383 mL | 0.8765 mL | 2.1913 mL | |
| 20 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6435 mL | |
| 25 mM | 0.0526 mL | 0.2630 mL | 0.5259 mL | 1.3148 mL | |
| 30 mM | 0.0438 mL | 0.2191 mL | 0.4383 mL | 1.0957 mL | |
| 40 mM | 0.0329 mL | 0.1644 mL | 0.3287 mL | 0.8218 mL | |
| DMSO | 50 mM | 0.0263 mL | 0.1315 mL | 0.2630 mL | 0.6574 mL |
| 60 mM | 0.0219 mL | 0.1096 mL | 0.2191 mL | 0.5478 mL | |
| 80 mM | 0.0164 mL | 0.0822 mL | 0.1644 mL | 0.4109 mL | |
| 100 mM | 0.0131 mL | 0.0657 mL | 0.1315 mL | 0.3287 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ponceau S Related Classifications
HY-12489 Related Classifications
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