6226-79-5
Chemical Structure
Ponceau S
Synonym(s): Acid Red 112
- CAS No.: 6226-79-5
- Formula:C22H12N4Na4O13S4
- Molecular Weight:760.57
IUPAC Name: sodium 3-hydroxy-4-((E)-(2-sulfonato-4-((E)-(4-sulfonatophenyl)diazenyl)phenyl)diazenyl)naphthalene-2,7-disulfonate
InChIKey: KQHKSGRIBYJYFX-WNDMICSESA-J
SMILES: O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O
Biological Activity: Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red[1][2][3].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Ponceau S | 98.0% | Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red. | ||||||||||||||||||||
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Ponceau S, BS | Both Ponceau S and Ponceau BS are synthetic dyes commonly used in biological research. They are commonly used as protein stains to visualize proteins in western blots and other protein detection analyses. Ponceau S is a red dye, while Ponceau BS is a blue shade of the same dye. Both dyes bind to positively charged amino acid residues in proteins for easy visualization. However, Ponceau S is more commonly used due to its higher sensitivity. | |||||||||||||||||||||
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References
- [1]. Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53. [Content Brief]
- [2]. Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020(3): 098459. [Content Brief]
- [3]. Romero-Calvo I, et al. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem. 2010 Jun 15;401(2):318-20. [Content Brief]
