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  3. Biochemical Assay Reagents
  4. Hydroxynaphthol Blue

Hydroxynaphthol Blue is an azo dye and serves as a metal indicator. Hydroxynaphthol Blue binds to specific metal ions to form stable complexes with distinct color and fluorescence properties. Hydroxynaphthol Blue is used for the visual monitoring of isothermal nucleic acid amplification results. A visible color difference appears between positive and negative nucleic acid amplification reactions, allowing result discrimination by the naked eye without opening the reaction tube.

For research use only. We do not sell to patients.

Hydroxynaphthol Blue

Hydroxynaphthol Blue Chemical Structure

CAS No. : 63451-35-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Solution
10 mM * 1 mL in Water In-stock
Solid
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250 mg In-stock
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Hydroxynaphthol Blue Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

Hydroxynaphthol Blue is an azo dye and serves as a metal indicator. Hydroxynaphthol Blue binds to specific metal ions to form stable complexes with distinct color and fluorescence properties. Hydroxynaphthol Blue is used for the visual monitoring of isothermal nucleic acid amplification results. A visible color difference appears between positive and negative nucleic acid amplification reactions, allowing result discrimination by the naked eye without opening the reaction tube[1].
In Vitro

Hydroxynaphthol Blue (240 μM; 3 min initial denaturation at 94 °C, 45 min amplification at 62 °C, 5 min reaction termination at 80 °C)-based closed-tube SRCA assay enables visual, on-site detection of the Ara h 1-encoding gene from peanut (Arachis hypogaea) with an absolute limit of detection of 6.25 × 100 fg/μL, high specificity against non-peanut foods, and strong performance in processed food samples[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hydroxynaphthol Blue Related Antibodies
Molecular Weight

620.47

Formula

C20H11N2Na3O11S3
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S(C1=C(O)C(/N=N/C2=C3C=CC=CC3=C(S(=O)(O[Na])=O)C=C2O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 16.67 mg/mL (26.87 mM; ultrasonic and adjust pH to 12 with 1 M NaOH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6117 mL 8.0584 mL 16.1168 mL
5 mM 0.3223 mL 1.6117 mL 3.2234 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.6117 mL 8.0584 mL 16.1168 mL 40.2920 mL
5 mM 0.3223 mL 1.6117 mL 3.2234 mL 8.0584 mL
10 mM 0.1612 mL 0.8058 mL 1.6117 mL 4.0292 mL
15 mM 0.1074 mL 0.5372 mL 1.0745 mL 2.6861 mL
20 mM 0.0806 mL 0.4029 mL 0.8058 mL 2.0146 mL
25 mM 0.0645 mL 0.3223 mL 0.6447 mL 1.6117 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Hydroxynaphthol Blue Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hydroxynaphthol Blue63451-35-4Biochemical Assay Reagentsisothermal nucleic acid amplificationArachis hypogaeasaltatory rolling circle amplificationAra h 1magnesium ionInhibitorinhibitorinhibit

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