Direct red 254 

Direct Red 254 is a bisazo dye for textile applications. Direct Red 254 can be used for amyloid tissue staining in basic research on neurodegenerative diseases (e.g., Alzheimer's disease)^[1][2].

Direct Red 254 (1% (w/v)) stains amyloid-like synthetic peptides and induces birefringence in vitro: some peptide fragments with amyloid-like properties (e.g., peptide fragment N14) exhibit green birefringence under polarized light after staining. This is a typical characteristic of amyloid deposits^[1].
Direct Red 254 can be effectively biodecolorized, and its decolorization efficiency is affected by its molecular structure and aggregation behavior in aqueous solution^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

571.49

C₂₂H₁₅N₅Na₂O₇S₂

6300-50-1

Solid

black

O=S(C1=C(N=NC2=CC=C(N=NC3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2)C(O)=C4C=CC(N)=CC4=C1)(O[Na])=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Hilbich C, et al. Amyloid-like properties of peptides flanking the epitope of amyloid precursor protein-specific monoclonal antibody 22C11. J Biol Chem. 1993;268(35):26571-26577.  [Content Brief]

  [2]. Brás R, et al. Monoazo and diazo dye decolourisation studies in a methanogenic UASB reactor. J Biotechnol. 2005;115(1):57-66.  [Content Brief]