Ponceau S, BS
Based on 1 Customer Validation
Both Ponceau S and Ponceau BS are synthetic dyes commonly used in biological research. They are commonly used as protein stains to visualize proteins in western blots and other protein detection analyses. Ponceau S is a red dye, while Ponceau BS is a blue shade of the same dye. Both dyes bind to positively charged amino acid residues in proteins for easy visualization. However, Ponceau S is more commonly used due to its higher sensitivity.
For research use only. We do not sell to patients.
- CAS No.: 6226-79-5
- Formula: C22H12N4Na4O13S4
- Molecular Weight:760.57
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Biological Activity
Ponceau S , BS is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6226-79-5
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Appearance Solid
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Molecular Weight 760.57
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Formula C22H12N4Na4O13S4
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Color Brown to reddish brown
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SMILES
O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O
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Synonyms
Acid Red 112, BS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL (65.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (65.74 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (246 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.3148 mL | 6.5740 mL | 13.1480 mL | 32.8701 mL |
| 5 mM | 0.2630 mL | 1.3148 mL | 2.6296 mL | 6.5740 mL | |
| 10 mM | 0.1315 mL | 0.6574 mL | 1.3148 mL | 3.2870 mL | |
| 15 mM | 0.0877 mL | 0.4383 mL | 0.8765 mL | 2.1913 mL | |
| 20 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6435 mL | |
| 25 mM | 0.0526 mL | 0.2630 mL | 0.5259 mL | 1.3148 mL | |
| 30 mM | 0.0438 mL | 0.2191 mL | 0.4383 mL | 1.0957 mL | |
| 40 mM | 0.0329 mL | 0.1644 mL | 0.3287 mL | 0.8218 mL | |
| 50 mM | 0.0263 mL | 0.1315 mL | 0.2630 mL | 0.6574 mL | |
| 60 mM | 0.0219 mL | 0.1096 mL | 0.2191 mL | 0.5478 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.