2. Anti-infection
  3. Enterovirus
  4. Brilliant Black BN

Brilliant Black BN  (Synonyms: E 151)

Cat. No.: HY-128382 Purity: ≥98.0%
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Brilliant black BN (E151) is an azo dye and a food colorant. Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease.

For research use only. We do not sell to patients.

Brilliant Black BN Chemical Structure

Brilliant Black BN Chemical Structure

CAS No. : 2519-30-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
10 mg USD 50 In-stock
25 mg USD 100 In-stock
50 mg USD 160 In-stock
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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Brilliant Black BN Related Antibodies

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Description

Brilliant black BN (E151) is an azo dye and a food colorant[1]. Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease[2].

IC50 & Target

IC50: 2.39 µM- 28.12 µM (various strains of EV71)[2]
In Vitro

Brilliant black BN (E151) inhibits the interaction between EV71 and its cellular uncoating factor cyclophilin A [1].
Brilliant black BN (E151) inhibited the infection EV71 -GFP at a concentration of 300 μM as evidenced from the reduced GFP signals. Brilliant black BN (E151)exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells, exhibits an IC50 value of 10.1 μM[1].
Brilliant black BN (E151) is able to inhibit all tested 28 EV71, CVA16 and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 μM to 28.12 μM ,whereas its 50% cytotoxic concentration is 1870 μM [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Brilliant Black BN Related Antibodies
In Vivo

Brilliant black BN (E151)(intraperitoneal injection; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group.All E151 270 treated mice are completely protected throughout the experiment[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 14-days old AG129 mice with EV71[1]
Dosage: 200 mg/kg
Administration: Intraperitoneal injection
Result: Had a Protective eddect on EV71 challenged AG129 mice by dye E151.
Molecular Weight

867.68

Formula

C28H17N5Na4O14S4
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S(C1=C2C=C(S(=O)(O[Na])=O)C(/N=N/C3=C4C=C(S(=O)(O[Na])=O)C=CC4=C(/N=N/C5=CC=C(S(=O)(O[Na])=O)C=C5)C=C3)=C(O)C2=C(NC(C)=O)C=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (144.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1525 mL 5.7625 mL 11.5250 mL
5 mM 0.2305 mL 1.1525 mL 2.3050 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1525 mL 5.7625 mL 11.5250 mL 28.8125 mL
5 mM 0.2305 mL 1.1525 mL 2.3050 mL 5.7625 mL
10 mM 0.1152 mL 0.5762 mL 1.1525 mL 2.8812 mL
15 mM 0.0768 mL 0.3842 mL 0.7683 mL 1.9208 mL
20 mM 0.0576 mL 0.2881 mL 0.5762 mL 1.4406 mL
25 mM 0.0461 mL 0.2305 mL 0.4610 mL 1.1525 mL
30 mM 0.0384 mL 0.1921 mL 0.3842 mL 0.9604 mL
40 mM 0.0288 mL 0.1441 mL 0.2881 mL 0.7203 mL
50 mM 0.0230 mL 0.1152 mL 0.2305 mL 0.5762 mL
60 mM 0.0192 mL 0.0960 mL 0.1921 mL 0.4802 mL
80 mM 0.0144 mL 0.0720 mL 0.1441 mL 0.3602 mL
100 mM 0.0115 mL 0.0576 mL 0.1152 mL 0.2881 mL
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Brilliant Black BN Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Brilliant Black BN2519-30-4E 151E151E-151EnterovirusRhinovirusHRVHRVsHEVHEVsEV71,infectionHFMDantiviral,foodadditive,contagiousdiseaseInhibitorinhibitorinhibit

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