2. Academic Validation
  3. Biosensing platform based on graphene oxide via self-assembly induced by synergic interactions

Biosensing platform based on graphene oxide via self-assembly induced by synergic interactions

  • Anal Biochem. 2014 Sep 1;460:16-21. doi: 10.1016/j.ab.2014.05.017.
Juan Tian 1,  Pei-Xin Yuan 2,  Dan Shan 3,  Shou-Nian Ding 4,  Guang-Yao Zhang 2,  Xue-Ji Zhang 2
Affiliations

Affiliations

  • 1 School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Key Laboratory of Environmental Materials & Environmental Engineering of Jiangsu Province, School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Department of Energy and Resources, Shuozhou Vocational and Technical College, Shouzhou 036002, China.
  • 2 School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
  • 3 School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Key Laboratory of Environmental Materials & Environmental Engineering of Jiangsu Province, School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China. Electronic address: [email protected].
  • 4 School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, China.
PMID: 24887416 DOI: 10.1016/j.ab.2014.05.017
Abstract

A novel self-assembled glucose biosensor based on graphene oxide (GO) was constructed by using 1-pyrenebutyric acid-N-hydroxysuccinimide ester (PANHS) as linking molecular. The stepwise self-assembly process was performed for PANHS anchoring in N,N-dimethylformamide (DMF) solvent and the further glucose oxidase (GOD) binding in aqueous solution, respectively. The molecular interactions and the morphologic properties were characterized by Fourier transform infrared spectroscopy (FTIR), field emission scanning electronic microscopy (FESEM), and atomic force microscopy (AFM). In addition, the quantitative loadings of anchored PANHS and GOD were well elucidated by surface plasmon resonance (SPR) measurements. The obtained novel glucose sensor exhibited satisfactory analytical performance to glucose: wide linear range (4.0×10(-6) to 4.4×10(-3) M), fast response (10s), high sensitivity (40.5±0.4 mA M(-1) cm(-2)), and low detection limit (2 μM, S/N=3). Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.

Keywords

Biosensor; Graphene oxide; Pyrenebutyric acid–N-hydroxysuccinimide ester (PANHS); Self-assembly.

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