1-Pyrenebutyric acid N-hydroxysuccinimide ester

Name: 1-Pyrenebutyric acid N-hydroxysuccinimide ester
Brand: MedChemExpress
SKU: HY-W013151
Rating: 4.5 (0 reviews)

Cat. No.: HY-W013151 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

1-Pyrenebutyric acid N-hydroxysuccinimide ester (PANHS) is a linker and interface coupling agent that can be used to prepare electrochemical biosensors. 1-Pyrenebutyric acid N-hydroxysuccinimide ester immobilizes SARS-CoV-2 spike antibodies on graphene sheets. 1-Pyrenebutyric acid N-hydroxysuccinimide ester is often used as an activation reagent for carboxylic acids in organic chemistry or biochemistry.

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1-Pyrenebutyric acid N-hydroxysuccinimide ester 화학구조

CAS No. : 114932-60-4

사이즈	재고	수량
무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
250 mg	해외재고보유	Estimated Time of Arrival: December 31
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	견적 받기
10 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

1-Pyrenebutyric acid N-hydroxysuccinimide ester (5 mM, 1 h) attaches the antibodies onto CNT-FET circuits in vitro^[3].
1-Pyrenebutyric acid N-hydroxysuccinimide ester is used as an interfacial coupling agent to immobilize SARS-CoV-2 spike antibodies on graphene sheets for the construction of field-effect transistor-based biosensor devices to detect SARS-CoV-2^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

385.41

화학식

C₂₄H₁₉NO₄

CAS No.

114932-60-4

Appearance

Solid

SMILES

O=C(ON1C(CCC1=O)=O)CCCC2=C(C3=C45)C=CC5=CC=CC4=CC=C3C=C2

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

용액&용해도

In Vitro:

DMSO : ≥ 100 mg/mL (259.46 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5946 mL	12.9732 mL	25.9464 mL
5 mM	0.5189 mL	2.5946 mL	5.1893 mL
10 mM	0.2595 mL	1.2973 mL	2.5946 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.93%

Select Batch:

Data Sheet (278 KB) SDS (394 KB)

COA (255 KB) HNMR (119 KB) RP-HPLC (277 KB)

Handling Instructions (2659 KB)

References

[1]. Benvidi A, et al. Comparison of impedimetric detection of DNA hybridization on the various biosensors based on modified glassy carbon electrodes with PANHS and nanomaterials of RGO and MWCNTs. Talanta. 2016 Jan 15;147:621-7. [Content Brief]

[2]. Tian J, et al. Biosensing platform based on graphene oxide via self-assembly induced by synergic interactions. Anal Biochem. 2014 Sep 1;460:16-21. [Content Brief]

[3]. Stefansson S, et al. Targeting antibodies to carbon nanotube field effect transistors by pyrene hydrazide modification of heavy chain carbohydrates[J]. Journal of Nanotechnology, 2012, 2012(1): 490175.

[4]. Fapyane D, et al. Graphitized-Carbon-Nanofiber Paper-Enzyme Electrode Fabrication Through Non-Covalent Modification for Enzyme Biofuel Cell Application. J Biomed Nanotechnol. 2015 Jan;11(1):137-42. [Content Brief]

[5]. Khandelwal V, et al. Graphene biosensor: Rapid and Accurate diagnostic method of COVID-19 (SARS-CoV-2). 2020.

[6]. Sakamoto H, et al. Development of biofuel cell using a complex of highly oriented immobilized his-tagged enzyme and carbon nanotube surface through a pyrene derivative. Journal of Nanoscience and Nanotechnology, 2019, 19(6): 3551-3557.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5946 mL	12.9732 mL	25.9464 mL	64.8660 mL
	5 mM	0.5189 mL	2.5946 mL	5.1893 mL	12.9732 mL
	10 mM	0.2595 mL	1.2973 mL	2.5946 mL	6.4866 mL
	15 mM	0.1730 mL	0.8649 mL	1.7298 mL	4.3244 mL
	20 mM	0.1297 mL	0.6487 mL	1.2973 mL	3.2433 mL
	25 mM	0.1038 mL	0.5189 mL	1.0379 mL	2.5946 mL
	30 mM	0.0865 mL	0.4324 mL	0.8649 mL	2.1622 mL
	40 mM	0.0649 mL	0.3243 mL	0.6487 mL	1.6216 mL
	50 mM	0.0519 mL	0.2595 mL	0.5189 mL	1.2973 mL
	60 mM	0.0432 mL	0.2162 mL	0.4324 mL	1.0811 mL
	80 mM	0.0324 mL	0.1622 mL	0.3243 mL	0.8108 mL
	100 mM	0.0259 mL	0.1297 mL	0.2595 mL	0.6487 mL