2. Anti-infection
  3. Influenza Virus
  4. Harringtonine

Harringtonine 

Cat. No.: HY-N0862 Purity: 99.93%
COA Handling Instructions

Harringtonine is a natural Cephalotaxus alkaloid that inhibits protein synthesis. Harringtonine has anti-chikungunya virus (CHIKV) activities with an EC50 of 0.24 μM.

For research use only. We do not sell to patients.

Harringtonine Chemical Structure

Harringtonine Chemical Structure

CAS No. : 26833-85-2

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 112 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 112 In-stock
Solid
5 mg USD 96 In-stock
10 mg USD 144 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 6 publication(s) in Google Scholar

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Description

Harringtonine is a natural Cephalotaxus alkaloid that inhibits protein synthesis. Harringtonine has anti-chikungunya virus (CHIKV) activities with an EC50 of 0.24 μM.

In Vitro

Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor site on the 60-S ribosome subunit and therefore block aminoacyl-tRNA binding and peptide bond formation[1]. Harringtonine displays potent inhibition of Chikungunya virus infection with an EC50 of 0.24 μM. Harringtonine could inhibit other alphaviruses[2]. Harringtonine inhibits the growth of human myeloid leukemia cells in vitro at low concentrations. The mechanism of the antitumor action of harringtonine is considered to be an effect on protein synthesis and is characterized by breakdown of polysomes to monosomes[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

531.59

Appearance

Solid

Formula

C28H37NO9
CAS No.
SMILES

O=C([[email protected]@](CC(OC)=O)(O)CCC(C)(O)C)O[[email protected]]1[[email protected]](C2=CC(OCO3)=C3C=C2CCN4CCC5)([H])[[email protected]]45C=C1OC
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (188.11 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8811 mL 9.4057 mL 18.8115 mL
5 mM 0.3762 mL 1.8811 mL 3.7623 mL
10 mM 0.1881 mL 0.9406 mL 1.8811 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.93%

COA (259 KB) HNMR (301 KB) LCMS (97 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[2]

For harringtonine treatment studies with Sindbis virus, BHK21 cells are seeded into 96-well plates and infected with Sindbis virus at an MOI of 1 for 1 h prior to being washed twice with PBS and incubated with various concentrations of harringtonine (0.1 μM, 1 μM, 5 μM, and 10 μM) at 37°C with 5% CO2. Cell supernatants are harvested for plaque assays at 24 h postinfection[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

Harringtonine26833-85-2Influenza VirusInhibitorinhibitorinhibit

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