1. Anti-infection
  2. Influenza Virus
  3. Harringtonine

Harringtonine 

Cat. No.: HY-N0862 Purity: 99.93%
Handling Instructions

Harringtonine is a natural Cephalotaxus alkaloid that inhibits protein synthesis. Harringtonine has anti-chikungunya virus (CHIKV) activities with an EC50 of 0.24 μM.

For research use only. We do not sell to patients.

Harringtonine Chemical Structure

Harringtonine Chemical Structure

CAS No. : 26833-85-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 112 In-stock
Estimated Time of Arrival: December 31
5 mg USD 96 In-stock
Estimated Time of Arrival: December 31
10 mg USD 144 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

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Description

Harringtonine is a natural Cephalotaxus alkaloid that inhibits protein synthesis. Harringtonine has anti-chikungunya virus (CHIKV) activities with an EC50 of 0.24 μM.

In Vitro

Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor site on the 60-S ribosome subunit and therefore block aminoacyl-tRNA binding and peptide bond formation[1]. Harringtonine displays potent inhibition of Chikungunya virus infection with an EC50 of 0.24 μM. Harringtonine could inhibit other alphaviruses[2]. Harringtonine inhibits the growth of human myeloid leukemia cells in vitro at low concentrations. The mechanism of the antitumor action of harringtonine is considered to be an effect on protein synthesis and is characterized by breakdown of polysomes to monosomes[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

531.59

Formula

C₂₈H₃₇NO₉

CAS No.
SMILES

O=C([[email protected]@](CC(OC)=O)(O)CCC(C)(O)C)O[[email protected]]1[[email protected]](C2=CC(OCO3)=C3C=C2CCN4CCC5)([H])[[email protected]]45C=C1OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (188.11 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8811 mL 9.4057 mL 18.8115 mL
5 mM 0.3762 mL 1.8811 mL 3.7623 mL
10 mM 0.1881 mL 0.9406 mL 1.8811 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

For harringtonine treatment studies with Sindbis virus, BHK21 cells are seeded into 96-well plates and infected with Sindbis virus at an MOI of 1 for 1 h prior to being washed twice with PBS and incubated with various concentrations of harringtonine (0.1 μM, 1 μM, 5 μM, and 10 μM) at 37°C with 5% CO2. Cell supernatants are harvested for plaque assays at 24 h postinfection[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.93%

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Keywords:

HarringtonineInfluenza VirusInhibitorinhibitorinhibit

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Harringtonine
Cat. No.:
HY-N0862
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