1. Membrane Transporter/Ion Channel
  2. ATP Synthase

Oligomycin A (Synonyms: MCH 32)

Cat. No.: HY-16589 Purity: 99.94%
Handling Instructions

Oligomycin A, created by Streptomyces, acts as a mitochondrial F0F1-ATPase inhibitor, with a Ki of 1 μM; Oligomycin A shows anti-fungal activity.

For research use only. We do not sell to patients.

Oligomycin A Chemical Structure

Oligomycin A Chemical Structure

CAS No. : 579-13-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 191 In-stock
Estimated Time of Arrival: December 31
1 mg USD 60 In-stock
Estimated Time of Arrival: December 31
5 mg USD 110 In-stock
Estimated Time of Arrival: December 31
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50 mg   Get quote  

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Description

Oligomycin A, created by Streptomyces, acts as a mitochondrial F0F1-ATPase inhibitor, with a Ki of 1 μM; Oligomycin A shows anti-fungal activity.

IC50 & Target

Ki: 1 μM (F0F1-ATPase)[1]

In Vitro

Oligomycin A is a mitochondrial F0F1-ATPase inhibitor with a Ki of 1 μM. Oligomycin A shows cytotoxic to the NCI-60 cell lines, with GI50 of 10 nM. Oligomycin A also inhibits the activity of Triton X-100-solubilized ATPase with a Ki of 0.1 μM. Furthermore, Oligomycin A has anti-fungal activity[1]. Oligomycin A (2.4 µM) inhibits the ability of spermatozoa to achieve feasible in vitro capacitation (IVC), and also suppresses progesterone-induced in vitro acrosome exocytosis (IVAE) as well as the concomitant peaks of O2 consumption and ATP levels[2].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (158.02 mM)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2641 mL 6.3206 mL 12.6413 mL
5 mM 0.2528 mL 1.2641 mL 2.5283 mL
10 mM 0.1264 mL 0.6321 mL 1.2641 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: 2.08 mg/mL (2.63 mM); Clear solution; Need warming

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (2.63 mM); Clear solution

References
Cell Assay
[1]

Drug (Oligomycin A, etc.) dilutions are added to monolayer or suspension cells in 96 well plates in triplicate for varying times. MTT is then added to the wells at a final concentration of 0.5 mg/mL. Supernatant is removed after pelleting the reduced MTT crystals. The crystals are fully dissolved in 40 mM HCl in isopropanol. Plates are scanned on a microplate reader at 595 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

791.06

Formula

C₄₅H₇₄O₁₁

CAS No.

579-13-5

SMILES

O=C(/C=C/[[email protected]](C)[[email protected]@H](O)[[email protected]](C)C([[email protected]](C)[[email protected]@H](O)[[email protected]@H]1C)=O)O[[email protected]@]([[email protected]@H]2C)([H])[[email protected]](C)[[email protected]@]3(CC[[email protected]](C)[[email protected]](C[[email protected]](O)C)O3)O[[email protected]@]2([H])CC[[email protected]@H](CC)/C=C/C=C/C[[email protected]@H](C)[[email protected]](O)[[email protected]@](C)(O)C1=O

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: 99.94%

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Product Name:
Oligomycin A
Cat. No.:
HY-16589
Quantity:

Oligomycin A

Cat. No.: HY-16589