Oligomycin A (Synonyms: MCH 32)

Name: Oligomycin A
Brand: MedChemExpress
SKU: HY-16589
Rating: 5.0 (51 reviews)

Cat. No.: HY-16589 Purity: 99.91%: Data Sheet
COA

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Oligomycin A (MCH 32), created by Streptomyces, acts as a mitochondrial F₀F₁-ATPase inhibitor, with a K_i of 1 μM; Oligomycin A shows anti-fungal activity.

For research use only. We do not sell to patients.

CAS No. : 579-13-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 51 publication(s) in Google Scholar

Other Forms of Oligomycin A:

Oligomycin In-stock
Oligomycin C In-stock
Oligomycin B In-stock
Oligomycin E Get quote
Oligomycin D Get quote
Oligomycin A (Standard) Get quote

51 Publications Citing Use of MCE Oligomycin A

Bio/Physico-chemical Assay

Cell Imaging/Staining

: Oligomycin A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 25;16(1):11018. [Abstract]; OCR traces of HEK293T cells treated with 10 µM bedaquiline (BDQ), ethanol vehicle control (EtOH), and 1.5 µM Oligomycin A for 5 min. Arrow indicates the time point at which drug was added.

: Oligomycin A purchased from MedChemExpress. Usage Cited in: Nat Protoc. 2024 May;19(5):1311-1347. [Abstract]; Quantitative analysis of FiLa expression in cytosol (Oligo, 1 μM).

: Oligomycin A purchased from MedChemExpress. Usage Cited in: Nat Protoc. 2024 May;19(5):1311-1347. [Abstract]; Fluorescent images of FiLa expressed in cytosol (Oligo, 1 μM).

: Oligomycin A purchased from MedChemExpress. Usage Cited in: Nat Protoc. 2024 May;19(5):1311-1347. [Abstract]; After sequentially injecting Glucose (Glc, 1 mM), Oligomycin (Oligo, 1 μM, 20 min), and 2-deoxyglucose (2-DG, 50 mM) into HEK-293 cells (n = 9), extracellular acidification rate (ECAR) was measured using a Seahorse XF96 analyzer to perform glycolytic stress testing.

: Oligomycin A purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Jan 3;35(1):200-211.e9. [Abstract]; Inhibition of oxidative phosphorylation using the complex I inhibitor rotenone or the complex V inhibitor oligomycin A (3.5 μM) resulted in increased extracellular and subcellular lactate levels due to compensatory upregulation of glycolysis.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Oligomycin A (MCH 32), created by Streptomyces, acts as a mitochondrial F₀F₁-ATPase inhibitor, with a K_i of 1 μM; Oligomycin A shows anti-fungal activity.

IC₅₀ & Target

Ki: 1 μM (F₀F₁-ATPase)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.9 μM Compound: Oligomycin A	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32942072]
HCT-116	IC₅₀	1 μM Compound: Oligomycin A	Antitumor activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antitumor activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32942072]
K562	IC₅₀	0.2 μM Compound: Oligomycin A	Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32942072]
K562	IC₅₀	0.2 μM Compound: Oligomycin A	Antitumor activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antitumor activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32942072]
SH-SY5Y	IC₅₀	29 μM Compound: Oligomycin A	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27689727]

In Vitro

Oligomycin A is a mitochondrial F₀F₁-ATPase inhibitor with a K_i of 1 μM. Oligomycin A shows cytotoxic to the NCI-60 cell lines, with GI₅₀ of 10 nM. Oligomycin A also inhibits the activity of Triton X-100-solubilized ATPase with a K_i of 0.1 μM. Furthermore, Oligomycin A has anti-fungal activity^[1]. Oligomycin A (2.4 μM) inhibits the ability of spermatozoa to achieve feasible in vitro capacitation (IVC), and also suppresses progesterone-induced in vitro acrosome exocytosis (IVAE) as well as the concomitant peaks of O₂ consumption and ATP levels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

791.06

Formula

C₄₅H₇₄O₁₁

CAS No.

579-13-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(/C=C/[C@H](C)[C@@H](O)[C@H](C)C([C@H](C)[C@@H](O)[C@@H]1C)=O)O[C@@]([C@@H]2C)([H])[C@H](C)[C@@]3(CC[C@H](C)[C@H](C[C@H](O)C)O3)O[C@@]2([H])CC[C@@H](CC)/C=C/C=C/C[C@@H](C)[C@H](O)[C@@](C)(O)C1=O

Structure Classification

Initial Source

Microorganisms

Streptomyces diastatochromogenes

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (126.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (126.41 mM; ultrasonic and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2641 mL	6.3206 mL	12.6413 mL
5 mM	0.2528 mL	1.2641 mL	2.5283 mL
10 mM	0.1264 mL	0.6321 mL	1.2641 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.16 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (279 KB) SDS (396 KB)

COA (256 KB) HNMR (247 KB) LCMS (336 KB) Elemental Analysis Report (113 KB)

Handling Instructions (2659 KB)

References

[1]. Salomon AR, et al. Apoptolidin, a selective cytotoxic agent, is an inhibitor of F0F1-ATPase. Chem Biol. 2001 Jan;8(1):71-80. [Content Brief]

[2]. Ramió-Lluch L, et al. Oligomycin A-induced inhibition of mitochondrial ATP-synthase activity suppresses boar sperm motility and in vitro capacitation achievement without modifying overall sperm energy levels. Reprod Fertil Dev. 2014;26(6):883-97. [Content Brief]

Cell Assay
^[1]

Drug (Oligomycin A, etc.) dilutions are added to monolayer or suspension cells in 96 well plates in triplicate for varying times. MTT is then added to the wells at a final concentration of 0.5 mg/mL. Supernatant is removed after pelleting the reduced MTT crystals. The crystals are fully dissolved in 40 mM HCl in isopropanol. Plates are scanned on a microplate reader at 595 nm^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Salomon AR, et al. Apoptolidin, a selective cytotoxic agent, is an inhibitor of F0F1-ATPase. Chem Biol. 2001 Jan;8(1):71-80. [Content Brief]

[2]. Ramió-Lluch L, et al. Oligomycin A-induced inhibition of mitochondrial ATP-synthase activity suppresses boar sperm motility and in vitro capacitation achievement without modifying overall sperm energy levels. Reprod Fertil Dev. 2014;26(6):883-97. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	1.2641 mL	6.3206 mL	12.6413 mL	31.6032 mL
	5 mM	0.2528 mL	1.2641 mL	2.5283 mL	6.3206 mL
	10 mM	0.1264 mL	0.6321 mL	1.2641 mL	3.1603 mL
	15 mM	0.0843 mL	0.4214 mL	0.8428 mL	2.1069 mL
	20 mM	0.0632 mL	0.3160 mL	0.6321 mL	1.5802 mL
	25 mM	0.0506 mL	0.2528 mL	0.5057 mL	1.2641 mL
	30 mM	0.0421 mL	0.2107 mL	0.4214 mL	1.0534 mL
	40 mM	0.0316 mL	0.1580 mL	0.3160 mL	0.7901 mL
	50 mM	0.0253 mL	0.1264 mL	0.2528 mL	0.6321 mL
	60 mM	0.0211 mL	0.1053 mL	0.2107 mL	0.5267 mL
	80 mM	0.0158 mL	0.0790 mL	0.1580 mL	0.3950 mL
	100 mM	0.0126 mL	0.0632 mL	0.1264 mL	0.3160 mL

Oligomycin A Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oligomycin A579-13-5MCH 32MCH32MCH-32ATP SynthaseFungalAntibioticInhibitorinhibitorinhibit

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Oligomycin A (Synonyms: MCH 32)

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Other Forms of Oligomycin A:

Oligomycin A Related Antibodies

51 Publications Citing Use of MCE Oligomycin A

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Purity & Documentation

References

Customer Review

Oligomycin A Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Oligomycin A Related Classifications

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