1. Autophagy
    Cell Cycle/DNA Damage
  2. Autophagy
    CRISPR/Cas9
    Mitophagy
  3. Brefeldin A

Brefeldin A (Synonyms: BFA; Cyanein; Decumbin)

Cat. No.: HY-16592 Purity: 99.79%
Handling Instructions

Brefeldin A is a specific inhibitor of protein trafficking which blocks the protein transport from the endoplasmic reticulum to the Golgi complex.

For research use only. We do not sell to patients.

Brefeldin A Chemical Structure

Brefeldin A Chemical Structure

CAS No. : 20350-15-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 72 In-stock
Estimated Time of Arrival: December 31
50 mg USD 240 In-stock
Estimated Time of Arrival: December 31
100 mg USD 440 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Brefeldin A purchased from MCE. Usage Cited in: J Radiat Res. 2017 Jul 1;58(4):474-486.

    Images showing the morphology of axons after 2.0 mT of 50 Hz MF exposure for 3 days, 1 h per day. Histograms showing the average axon length per neuron, the average axon branch length per neuron and the average number of branches per axon in the sham and exposure groups. Neurons were treated with 1.0 µg/mL brefeldin A at DIV2 as a positive control.

    Brefeldin A purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.

    Apoptotic effects induced by BFA are examined in CRC cells.

    Brefeldin A purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.

    The interaction of Beclin 1 with Bcl-2 or ATG14L after BFA treatment is determined by coimmunoprecipitation assay.

    Brefeldin A purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.

    CRC cells are treated with vehicle, BFA (100 nM), 3-MA (10 mM), or in combination for 24 h. Immunoblot analysis is used to detect LC3 turnover.

    Brefeldin A purchased from MCE. Usage Cited in: J Cell Mol Med. 2019 Apr;23(4):2399-2409.

    MHCC-97H cells are treated with BFA (2.5 μg/mL) for 0, 0.5, 1, 2, 6, and 12 h. The expression of GP73 and intracellular MMP-2 is measured using immunoblotting.
    • Biological Activity

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    • References

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    Description

    Brefeldin A is a specific inhibitor of protein trafficking which blocks the protein transport from the endoplasmic reticulum to the Golgi complex.

    IC50 & Target[5]

    CRISPR/Cas9

     

    In Vitro

    Brefeldin A treatment for 15 h or 40 h, causes dramatic swelling of the Endoplasmic Reticulum (ER) and shifts its localization to the periphery of normal rat kidney (NRK) cells. Prolonged Brefeldin A treatment results in marked disruption of the MT and actin cytoskeleton[1]. ADP-ribosylation of BARS is mediated by formation of a conjugate between Brefeldin A and ADPR. BARS shows BAC binding when incubated with the medium from the BFA-treated CD38+ HeLa cells[2]. Brefeldin A induces anchorage-independent cell death in MDA-MB-231 breast cancer cells, inhibits the formation of MDA-MB-231 colonies in 3D and 2D cultures and inhibits the migration and MMP 9 (Matrix Metallopeptidase 9) activity of MDA-MB-231[3].

    Molecular Weight

    280.36

    Formula

    C₁₆H₂₄O₄

    CAS No.

    20350-15-6

    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 20.83 mg/mL (74.30 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5668 mL 17.8342 mL 35.6684 mL
    5 mM 0.7134 mL 3.5668 mL 7.1337 mL
    10 mM 0.3567 mL 1.7834 mL 3.5668 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (7.42 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (7.42 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (7.42 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    Cells are grown on glass coverslips, fixed in 3 % paraformaldehyde in PBS (10 min at room temperature) and then washed in PBS. Cells are permeabilized with 0.01 % Triton X-lOO in PBS at room temperature for 7 min. The coverslips are washed (3 times in PBS/0.2 % Tween) incubated in PBS/O.4 % fish skin gelatin/0.2 % Tween (5 min) and in PBS/2.5 % goat serum/0.2 % Tween (5 min.). After blocking, the cells are incubated with primary antibodies for 45 min at 37°C, and then washed with PBS/0.2 % Tween (5 times, 5 min each). The secondary antibodies are added for 30 min at 37°C and then cells are washed as above. Coverslips are mounted on slides in 9: 1 glycerol/PBS with 0.1 % o-phenylenediamine.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.79%

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    Product Name:
    Brefeldin A
    Cat. No.:
    HY-16592
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