1. Cell Cycle/DNA Damage
    Autophagy
  2. CRISPR/Cas9
    Autophagy

Brefeldin A (Synonyms: Ascotoxin; Cyanein; Decumbin; Nectrolide; BFA; Synergisidin)

Cat. No.: HY-16592 Purity: 99.54%
Handling Instructions

Brefeldin A is an alternative autophagy inhibitor, and also increases CRISPR/Cas9-mediated editing frequencies.

For research use only. We do not sell to patients.
Brefeldin A Chemical Structure

Brefeldin A Chemical Structure

CAS No. : 20350-15-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
5 mg USD 60 In-stock
10 mg USD 72 In-stock
50 mg USD 168 In-stock
100 mg USD 264 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

    Brefeldin A purchased from MCE. Usage Cited in: J Radiat Res. 2017 Jul 1;58(4):474-486.

    Images showing the morphology of axons after 2.0 mT of 50 Hz MF exposure for 3 days, 1 h per day. Histograms showing the average axon length per neuron, the average axon branch length per neuron and the average number of branches per axon in the sham and exposure groups. Neurons were treated with 1.0 µg/mL brefeldin A at DIV2 as a positive control.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Brefeldin A is an alternative autophagy inhibitor, and also increases CRISPR/Cas9-mediated editing frequencies.

    IC50 & Target

    Autophagy[4], CRISPR/Cas9[5]

    In Vitro

    Brefeldin A treatment for 15 h or 40 h, causes dramatic swelling of the Endoplasmic Reticulum (ER) and shifts its localization to the periphery of normal rat kidney (NRK) cells. Prolonged Brefeldin A treatment results in marked disruption of the MT and actin cytoskeleton[1]. ADP-ribosylation of BARS is mediated by formation of a conjugate between Brefeldin A and ADPR. BARS shows BAC binding when incubated with the medium from the BFA-treated CD38+ HeLa cells[2]. Brefeldin A induces anchorage-independent cell death in MDA-MB-231 breast cancer cells, inhibits the formation of MDA-MB-231 colonies in 3D and 2D cultures and inhibits the migration and MMP 9 (Matrix Metallopeptidase 9) activity of MDA-MB-231[3].

    Clinical Trial
    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 3.5668 mL 17.8342 mL 35.6684 mL
    5 mM 0.7134 mL 3.5668 mL 7.1337 mL
    10 mM 0.3567 mL 1.7834 mL 3.5668 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [1]

    Cells are grown on glass coverslips, fixed in 3 % paraformaldehyde in PBS (10 min at room temperature) and then washed in PBS. Cells are permeabilized with 0.01 % Triton X-lOO in PBS at room temperature for 7 min. The coverslips are washed (3 times in PBS/0.2 % Tween) incubated in PBS/O.4 % fish skin gelatin/0.2 % Tween (5 min) and in PBS/2.5 % goat serum/0.2 % Tween (5 min.). After blocking, the cells are incubated with primary antibodies for 45 min at 37°C, and then washed with PBS/0.2 % Tween (5 times, 5 min each). The secondary antibodies are added for 30 min at 37°C and then cells are washed as above. Coverslips are mounted on slides in 9: 1 glycerol/PBS with 0.1 % o-phenylenediamine. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    280.36

    Formula

    C₁₆H₂₄O₄

    CAS No.

    20350-15-6

    SMILES

    O[[email protected]@H]1C[[email protected]]2([H])[[email protected]@](/C=C/CCC[[email protected]](C)OC(/C=C/[[email protected]]2O)=O)([H])C1

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 28 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: 99.54%

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    Inquiry Information

    Product Name:
    Brefeldin A
    Cat. No.:
    HY-16592
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