2. Membrane Transporter/Ion Channel Anti-infection Apoptosis
  3. ATP Synthase Bacterial Apoptosis Antibiotic
  4. Oligomycin B

Oligomycin B is an antibiotic that acts as a non-selective inhibitor of ATP Synthase. Oligomycin B increases mitochondrial membrane potential. Oligomycin B induces apoptosis and necrosis. Oligomycin B impairs the motility of Plasmopara viticola zoospores and induces their lysis. Oligomycin B inhibits Magnaporthe oryzae (wheat blast fungus) and suppresses the development of wheat blast. Oligomycin B reduces hyphal growth and spore germination of Botrytis cinerea, and protects Arabidopsis thaliana against Botrytis cinerea infection. Oligomycin B exacerbates cytotoxic brain edema in rats with cerebral cortical contusion, increases intracranial pressure and brain water content, and aggravates mitochondrial damage in these rats. Oligomycin B is used in studies related to grape downy mildew, traumatic brain injury, wheat blast, and gray mold.

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Oligomycin B

Oligomycin B Chemical Structure

CAS No. : 11050-94-5

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Based on 1 publication(s) in Google Scholar

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Description

Oligomycin B is an antibiotic that acts as a non-selective inhibitor of ATP Synthase. Oligomycin B increases mitochondrial membrane potential. Oligomycin B induces apoptosis and necrosis. Oligomycin B impairs the motility of Plasmopara viticola zoospores and induces their lysis. Oligomycin B inhibits Magnaporthe oryzae (wheat blast fungus) and suppresses the development of wheat blast. Oligomycin B reduces hyphal growth and spore germination of Botrytis cinerea, and protects Arabidopsis thaliana against Botrytis cinerea infection. Oligomycin B exacerbates cytotoxic brain edema in rats with cerebral cortical contusion, increases intracranial pressure and brain water content, and aggravates mitochondrial damage in these rats. Oligomycin B is used in studies related to grape downy mildew, traumatic brain injury, wheat blast, and gray mold[1][2][3][4][5].

In Vitro

Oligomycin B promotes the formation of JC-1 J-aggregates and increases the mitochondrial membrane potential of P388D1 cells[1].
Oligomycin B (20-50 μM; 24 h) induces apoptosis (at low concentrations) and predominantly necrosis (at high concentrations) in P388D1 cells, with an apoptosis rate of 25% observed at the concentration of 20 μM[1].
Oligomycin B (0-0.5 μg/mL; 0-60 min) impairs the motility of Plasmopara viticola zoospores and induces their lysis in a dose- and time-dependent manner, with corresponding IC50 values of 0.15 and 0.2 μg/mL; complete inhibition of zoospore motility and induction of lysis are achieved at concentrations as low as 0.25 μg/mL (after 60 min of treatment) or 0.5 μg/mL (after 30 min of treatment)[2].
Oligomycin B (0.05-2 μg/disk) inhibits the mycelial growth of the wheat-specialized isolate BTJP of *Magnaporthe oryzae* in a dose-dependent manner, with an inhibition rate of 8.63% at 0.05 μg/disk and 57.1% at 2 μg/disk, and also induces abnormal mycelial morphology[4].
Oligomycin B (5-100 μg/mL; 24 h) inhibits conidiation of Magnaporthe oryzae Triticum[4].
Oligomycin B (0.05 μg/mL; 6-24 h) inhibits conidial germination of Magnaporthe oryzae Triticum, induces conidial lysis and abnormal germ tube development, and completely blocks its germination and subsequent growth within 24 h[4].
Oligomycin B (10-150 μg/disc; 3 days) inhibits mycelial growth of Botrytis cinerea in the disc diffusion assay, with a minimum inhibitory concentration of 10 µg/disc[5].
Oligomycin B (5-20 µg/mL; 24 h) delays or inhibits spore germination of Botrytis cinerea in a concentration-dependent manner. It achieves complete inhibition at 20 µg/mL, while exerting a strong delaying effect at 10 µg/mL[5].
Oligomycin B (5-100 μg/mL) dose-dependently inhibits the development of wheat blast lesions on detached wheat leaves, and achieves complete inhibition at 100 μg/mL[4].
Oligomycin B (75% cell-free filtrate of Streptomyces sp. S5.1) significantly reduces the lesion size of gray mold in Arabidopsis thaliana, with average lesion sizes decreased by approximately 61% and 58%, respectively[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Oligomycin B Related Antibodies

Apoptosis Analysis[1]

Cell Line: P388D1 cells
Concentration: 20 μM; 50 μM
Incubation Time: 24 h
Result: Induced 25% apoptosis in P388D1 cells at 20 μM.
Induced some apoptosis but primarily necrosis in P388D1 cells at 50 μM.
In Vivo

Oligomycin B (0.25 mg/kg; i.p.; two doses at 30 min and 24 h post-injury) significantly worsens post-traumatic cerebral edema, intracranial pressure elevation, mitochondrial dysfunction, and neuronal/axonal damage in male Sprague-Dawley rats with traumatic brain injury[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley (male, 250-300 g, traumatic brain injury modified impact acceleration model)[3]
Dosage: 0.25 mg/kg
Administration: i.p.; two doses at 30 min and 24 h post-injury
Result: Increased intracranial pressure to levels 2.5-fold higher than cyclosporine A-treated rats.
Significantly increased brain water content compared to cyclosporine A-treated rats.
Caused significantly greater mitochondrial transmembrane potential loss than vehicle-treated rats.
Induced severely swollen neurons with a significantly elevated cytoplasm/nucleus area ratio, and profoundly worsened axonal damage with numerous blown-up mitochondria in severely swollen axons.
Molecular Weight

805.05

Formula

C45H72O12
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H]([C@H]([C@@H](C([C@@](C)([C@H]([C@@H](C/C=C/C=C/[C@@]([H])(CC[C@]1([C@H]([C@@]([H])(O2)[C@@H]([C@]3(O1)C(C[C@@H]([C@@]([H])(O3)C[C@@H](C)O)C)=O)C)C)[H])CC)C)O)O)=O)C)O)C)[C@H]([C@@H]([C@H](/C=C/C2=O)C)O)C
Structure Classification
Initial Source

the marine Streptomyces strains B8496 and B8739
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (62.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2422 mL 6.2108 mL 12.4216 mL
5 mM 0.2484 mL 1.2422 mL 2.4843 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (1.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 97.67%

SDS (394 KB)

COA (258 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2422 mL 6.2108 mL 12.4216 mL 31.0540 mL
5 mM 0.2484 mL 1.2422 mL 2.4843 mL 6.2108 mL
10 mM 0.1242 mL 0.6211 mL 1.2422 mL 3.1054 mL
15 mM 0.0828 mL 0.4141 mL 0.8281 mL 2.0703 mL
20 mM 0.0621 mL 0.3105 mL 0.6211 mL 1.5527 mL
25 mM 0.0497 mL 0.2484 mL 0.4969 mL 1.2422 mL
30 mM 0.0414 mL 0.2070 mL 0.4141 mL 1.0351 mL
40 mM 0.0311 mL 0.1553 mL 0.3105 mL 0.7763 mL
50 mM 0.0248 mL 0.1242 mL 0.2484 mL 0.6211 mL
60 mM 0.0207 mL 0.1035 mL 0.2070 mL 0.5176 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oligomycin B11050-94-5ATP SynthaseBacterialApoptosisAntibioticBotrytis cinereaArabidopsis thalianamitochondrial ATP synthasewheat blastratsPlasmopara viticolaMagnaporthe oryzae Triticumcerebral cytotoxic edemaP388D1 cellstraumatic brain injuryantibioticgrape downy mildewgray moldInhibitorinhibitorinhibit

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