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  4. Bromobimane

Bromobimane  (Synonyms: Monobromobimane)

Cat. No.: HY-100041 Purity: 99.31%
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Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols.

For research use only. We do not sell to patients.

CAS No. : 71418-44-5

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Based on 8 publication(s) in Google Scholar

Other Forms of Bromobimane:

Bromobimane Related Antibodies

Top Publications Citing Use of Products

    Bromobimane purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Apr 15:693:137611.  [Abstract]

    Fluorescence images of the orthotopic liver cancer tumor slices stained with the GSH probe (Bromobimane (blue) collected 24 h after injection of the various agents

    Bromobimane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2022 Nov 3;193(Pt 1):459-473.  [Abstract]

    DHE and Bromobimane (MBB: 20 ​​μM) staining revealed the levels of ROS accumulation and GSH content in TCMK-1 cells.

    Bromobimane purchased from MedChemExpress. Usage Cited in: Cell Stress Chaperones. 2023 Jan;28(1):91-103.  [Abstract]

    The condition of intracellular GSH levels assessed by Bromobimane (MBB: 50 μM for 30 min) staining.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols[1][2].

    In Vitro

    Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
    Bromobimane (mBBr) labeled reactive thiols in cells[1]
    (1) Prepare a 100 mM mBBr solution with acetonitrile.
    (2) Add 1 mL of 10% cell suspension to 15-25 μL of 100 mM mBBr solution.
    (3) After incubation at 37°C for 30-45 min, fluorescence detection was performed.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Bromobimane Related Antibodies
    Molecular Weight

    271.11

    Formula

    C10H11BrN2O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    Emission (Em)

    450
    Excitation (Ex)

    344
    SMILES

    O=C1N(C(C(C)=C2C)=O)N2C(CBr)=C1C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 87.5 mg/mL (322.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 1 mg/mL (3.69 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6885 mL 18.4427 mL 36.8854 mL
    5 mM 0.7377 mL 3.6885 mL 7.3771 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.31%

    SDS (394 KB)

    COA (255 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.6885 mL 18.4427 mL 36.8854 mL 92.2135 mL
    DMSO 5 mM 0.7377 mL 3.6885 mL 7.3771 mL 18.4427 mL
    10 mM 0.3689 mL 1.8443 mL 3.6885 mL 9.2213 mL
    15 mM 0.2459 mL 1.2295 mL 2.4590 mL 6.1476 mL
    20 mM 0.1844 mL 0.9221 mL 1.8443 mL 4.6107 mL
    25 mM 0.1475 mL 0.7377 mL 1.4754 mL 3.6885 mL
    30 mM 0.1230 mL 0.6148 mL 1.2295 mL 3.0738 mL
    40 mM 0.0922 mL 0.4611 mL 0.9221 mL 2.3053 mL
    50 mM 0.0738 mL 0.3689 mL 0.7377 mL 1.8443 mL
    60 mM 0.0615 mL 0.3074 mL 0.6148 mL 1.5369 mL
    80 mM 0.0461 mL 0.2305 mL 0.4611 mL 1.1527 mL
    100 mM 0.0369 mL 0.1844 mL 0.3689 mL 0.9221 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Bromobimane Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bromobimane71418-44-5MonobromobimaneFluorescent DyeInhibitorinhibitorinhibit

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