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  4. BODIPY-FL NHS ester

BODIPY-FL NHS ester  (Synonyms: BODIPY FL,SE)

Cat. No.: HY-110210 Purity: 99.32%
COA Handling Instructions

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BODIPY-FL NHS ester (BODIPY FL, SE) is an amine-reactive fluorescent probe. The maximum excitation/emission values are 502/511 nm. BODIPY-FL NHS ester can be used to synthesize protease substrates.

For research use only. We do not sell to patients.

BODIPY-FL NHS ester Chemical Structure

BODIPY-FL NHS ester Chemical Structure

CAS No. : 146616-66-2

Size Price Stock Quantity
1 mg USD 100 In-stock
5 mg USD 180 In-stock
10 mg USD 280 In-stock
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Based on 1 publication(s) in Google Scholar

BODIPY-FL NHS ester Related Antibodies

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Description

BODIPY-FL NHS ester (BODIPY FL, SE) is an amine-reactive fluorescent probe. The maximum excitation/emission values are 502/511 nm. BODIPY-FL NHS ester can be used to synthesize protease substrates[1][2].

Molecular Weight

389.16

Formula

C18H18BF2N3O4
CAS No.
Appearance

Solid

Color

Orange to red

Emission (Em)

510
Excitation (Ex)

502
SMILES

O=C(ON(C1=O)C(CC1)=O)CCC2=CC=C(C=C(C(C)=C3)[N]4=C3C)[N-]2[B+3]4([F-])[F-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (321.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5696 mL 12.8482 mL 25.6964 mL
5 mM 0.5139 mL 2.5696 mL 5.1393 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.32%

COA (255 KB) HNMR (269 KB) LCMS (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5696 mL 12.8482 mL 25.6964 mL 64.2409 mL
5 mM 0.5139 mL 2.5696 mL 5.1393 mL 12.8482 mL
10 mM 0.2570 mL 1.2848 mL 2.5696 mL 6.4241 mL
15 mM 0.1713 mL 0.8565 mL 1.7131 mL 4.2827 mL
20 mM 0.1285 mL 0.6424 mL 1.2848 mL 3.2120 mL
25 mM 0.1028 mL 0.5139 mL 1.0279 mL 2.5696 mL
30 mM 0.0857 mL 0.4283 mL 0.8565 mL 2.1414 mL
40 mM 0.0642 mL 0.3212 mL 0.6424 mL 1.6060 mL
50 mM 0.0514 mL 0.2570 mL 0.5139 mL 1.2848 mL
60 mM 0.0428 mL 0.2141 mL 0.4283 mL 1.0707 mL
80 mM 0.0321 mL 0.1606 mL 0.3212 mL 0.8030 mL
100 mM 0.0257 mL 0.1285 mL 0.2570 mL 0.6424 mL
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BODIPY-FL NHS ester Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY-FL NHS ester146616-66-2BODIPY FL,SEFluorescent DyeBODIPYLX2 cellsFluorescent probesLive cell imagingVascular smooth muscle cellsInhibitorinhibitorinhibit

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