Bioconversion of rebaudioside I from rebaudioside A

Molecules. 2014 Oct 28;19(11):17345-55. doi: 10.3390/molecules191117345.

Indra Prakash ¹, Cynthia Bunders ², Krishna P Devkota ³, Romila D Charan ⁴, Catherine Ramirez ⁵, Tara M Snyder ⁶, Christopher Priedemann ⁷, Avetik Markosyan ⁸, Cyrille Jarrin ⁹, Robert Ter Halle ¹⁰

Affiliations

1 The Coca-Cola Company, Atlanta, GA 30313, USA. [email protected].
2 The Coca-Cola Company, Atlanta, GA 30313, USA. [email protected].
3 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. [email protected].
4 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. [email protected].
5 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. [email protected].
6 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. [email protected].
7 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. [email protected].
8 PureCircle Limited, Lengkuk Teknologi, Bandar Enstek 71760, Malaysia. [email protected].
9 Libragen SA, 3 Rue des Satellites, Toulouse 31400, France. [email protected].
10 Libragen SA, 3 Rue des Satellites, Toulouse 31400, France. [email protected].

PMID: 25353385 DOI: 10.3390/molecules191117345

Abstract

To supply the increasing demand of natural high potency Sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase Enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.

Products

Cat. No.

Product Name

Description

Target

Research Area
HY-N6889

Rebaudioside I

98.33%

Others

Others

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