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  3. Deoxypodophyllotoxin induces G2/M cell cycle arrest and apoptosis in SGC-7901 cells and inhibits tumor growth in vivo

Deoxypodophyllotoxin induces G2/M cell cycle arrest and apoptosis in SGC-7901 cells and inhibits tumor growth in vivo

  • Molecules. 2015 Jan 20;20(1):1661-75. doi: 10.3390/molecules20011661.
Yu-Rong Wang 1,  Yuan Xu 2,  Zhen-Zhou Jiang 3,  Mounia Guerram 4,  Bin Wang 5,  Xiong Zhu 6,  Lu-Yong Zhang 7
Affiliations

Affiliations

  • 1 Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. [email protected].
  • 2 Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. [email protected].
  • 3 Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. [email protected].
  • 4 Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. [email protected].
  • 5 Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. [email protected].
  • 6 Medical and Chemical Institute, China Pharmaceutical University, Nanjing 210009, China. [email protected].
  • 7 Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. [email protected].
PMID: 25608854 DOI: 10.3390/molecules20011661
Abstract

Deoxypodophyllotoxin (DPT), a natural microtubule destabilizer, was isolated from Anthriscus sylvestris, and a few studies have reported its anti-cancer effect. However, the in vivo antitumor efficacy of DPT is currently indeterminate. In this study, we investigated the anti-gastric Cancer effects of DPT both in vitro and in vivo. Our data showed that DPT inhibited Cancer cell proliferation and induced G2/M cell cycle arrest accompanied by an increase in apoptotic cell death in SGC-7901 Cancer cells. In addition, DPT caused cyclin B1, Cdc2 and Cdc25C to accumulate, decreased the expression of Bcl-2 and activated Caspase-3 and PARP, suggesting that caspase-mediated pathways were involved in DPT-induced Apoptosis. Animal studies revealed that DPT significantly inhibited tumor growth and decreased microvessel density (MVD) in a xenograft model of gastric Cancer. Taken together, our findings provide a framework for further exploration of DPT as a novel chemotherapeutic for human gastric Cancer.

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