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  3. Spectroscopic studies on the interaction between anthragallol and DNA using of ethidium bromide as a fluorescence probe

Spectroscopic studies on the interaction between anthragallol and DNA using of ethidium bromide as a fluorescence probe

  • Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 15;141:239-43. doi: 10.1016/j.saa.2015.01.049.
Yan Gao 1,  Junsheng Li 2,  Guoxia Huang 1,  Liujuan Yan 1,  Zhen Dong 1
Affiliations

Affiliations

  • 1 Guangxi University of Science and Technology, Biological and Chemical Engineering, Liu Zhou 545006, China.
  • 2 Guangxi University of Science and Technology, Biological and Chemical Engineering, Liu Zhou 545006, China. Electronic address: [email protected].
PMID: 25681808 DOI: 10.1016/j.saa.2015.01.049
Abstract

The interaction of DNA with anthragallol (Ant) was investigated using ethidium bromide (EB) as a fluorescence probe, and the binding mechanism of Ant with DNA was researched via viscosity measurements. The results indicate that there is a complex of Ant and DNA, as confirmed by Ultraviolet visible absorption spectroscopy (UV-vis), Fluorescent and Resonance LIGHT Scattering spectrum (RLS) and viscosity measurements. Ant molecules could intercalate with the base pairs of DNA as evidenced by the hyperchromic effect of absorption spectra, the relative viscosity of DNA and significant increases in the melting temperature. The binding constants of Ant and DNA were obtained by the fluorescence quenching technique. Furthermore, the binding mechanisms of the reaction of Ant with DNA were also investigated. The RLS assay successfully evaluated the saturated value and measured the potential toxicity of Ant. Adriamycin, chrysophanol, rhein, and alizarin can be used as references to build a method based on the mechanism of interactions with DNA and the DNA-saturation binding value to rapidly evaluate the potential toxicity of Ant.

Keywords

Action mode; Anthragallol; DNA; Ethidium bromide; Intercalation; Saturation value.

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