2. Academic Validation
  3. LPLRFamide exerts short-term anorexigenic effects that coincide with magnocellular division of the hypothalamic paraventricular nucleus activation

LPLRFamide exerts short-term anorexigenic effects that coincide with magnocellular division of the hypothalamic paraventricular nucleus activation

  • Gen Comp Endocrinol. 2017 May 15;246:116-119. doi: 10.1016/j.ygcen.2016.06.023.
Amy Scheel 1,  Nick Deal 2,  Natalie St John 3,  Amy Wells 4,  Maggie Caruso 2,  Elizabeth R Gilbert 3,  Mark Cline 5
Affiliations

Affiliations

  • 1 Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
  • 2 Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
  • 3 Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
  • 4 Department of Psychology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
  • 5 Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA. Electronic address: [email protected].
PMID: 27318277 DOI: 10.1016/j.ygcen.2016.06.023
Abstract

LPLRFamide is a member of the RFamide peptide family that elicits an anorexigenic effect when centrally injected in chicks although the mechanism mediating this response is poorly understood. Therefore, the purpose of this experiment was to elucidate the hypothalamic mechanism of short-term anorexia after central administration of LPLRFamide in chicks. In Experiment 1 chicks centrally injected with LPLRFamide decreased food intake at 15min but not 30min following administration compared to vehicle-injected chicks. For Experiment 2, c-Fos immunoreactivity was quantified in several appetite-associated hypothalamic nuclei and in LPLRF-injected chicks, compared to vehicle-injected chicks, there was an increase in the number of reactive cells in the magnocellular division of the paraventricular nucleus. Lastly in Experiment 3, real time-PCR was performed and hypothalamic proopiomelanocortin (POMC) mRNA abundance was increased in LPRLFamide-injected chicks compared to vehicle-injected chicks. Thus, following central injection of LPLRFamide there is activation of the paraventricular nucleus of the hypothalamus and increased expression of hypothalamic POMC mRNA in chicks.

Keywords

Anorexigenic; Chick; Hypothalamus; LPLRFamide.

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