2. Academic Validation
  3. Ratiometric Fluorescent Probe for Lysosomal pH Measurement and Imaging in Living Cells Using Single-Wavelength Excitation

Ratiometric Fluorescent Probe for Lysosomal pH Measurement and Imaging in Living Cells Using Single-Wavelength Excitation

  • Anal Chem. 2017 Jul 5;89(13):7038-7045. doi: 10.1021/acs.analchem.7b00754.
Xingjiang Liu 1,  Yuanan Su 1,  Huihui Tian 1,  Lei Yang 1,  Hongyan Zhang 2,  Xiangzhi Song 1 3,  James W Foley 4
Affiliations

Affiliations

  • 1 College of Chemistry and Chemical Engineering, Central South University , Changsha 410083, China.
  • 2 Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
  • 3 State Key Laboratory of Fine Chemicals, Dalian University of Technology , Dalian 116024, China.
  • 4 Rowland Institute at Harvard, Harvard University , Cambridge, Massachusetts 02142, United States.
PMID: 28553716 DOI: 10.1021/acs.analchem.7b00754
Abstract

A novel lysosome-targeting ratiometric fluorescent probe (CQ-Lyso) based on the chromenoquinoline chromorphore has been developed for the selective and sensitive detection of intracellular pH in living cells. In acidic media, the protonation of the quinoline ring of CQ-Lyso induces an enhanced intramolecular charge transfer (ICT) process, which results in large red-shifts in both the absorption (104 nm) and emission (53 nm) spectra which forms the basis of a new ratiometric fluorescence pH sensor. This probe efficiently stains lysosomes with high Pearson's colocalization coefficients using LysoTrackerDeep Red (0.97) and LysoTrackerBlue DND-22 (0.95) as references. Importantly, we show that CQ-Lyso quantitatively measures and images lysosomal pH values in a ratiometric manner using single-wavelength excitation.

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