2. Academic Validation
  3. Constituents from Scutellaria barbata Inhibiting Nitric Oxide Production in LPS-Stimulated Microglial Cells

Constituents from Scutellaria barbata Inhibiting Nitric Oxide Production in LPS-Stimulated Microglial Cells

  • Chem Biodivers. 2017 Nov;14(11). doi: 10.1002/cbdv.201700231.
So Ra Lee 1,  Min-Suk Kim 2,  Sunggun Kim 2,  Kwang Woo Hwang 3,  So-Young Park 1 2
Affiliations

Affiliations

  • 1 Department of Nanomedicine, World Class University, Dankook University, Cheonan, 31116, Korea.
  • 2 Laboratory of Pharmacognosy, College of Pharmacy, Dankook University, Cheonan, 31116, Korea.
  • 3 College of Pharmacy, Chung-Ang University, Seoul, 06974, Korea.
PMID: 28805952 DOI: 10.1002/cbdv.201700231
Abstract

The arial parts of Scutellaria barbata D. Don (Lamiaceae) efficiently inhibited NO production in BV2 microglial cells, and the active constituents were further isolated based on activity-guided isolation using silica-gel column chromatography, RP-C18 MPLC and prep-HPLC. As the results, 2 Flavonoids including 6-methoxynaringenin (1) and 6-O-methylscutellarein (5), and 6 neo-clerodane diterpenes such as scutebarbatine W (2), scutebatas B (3), scutebarbatine B (4), scutebarbatine A (6), 6-O-nicotinolylscutebarbatine G (7), and scutebarbatine X (8) were isolated. The structures of these compounds were elucidated based on NMR and MS data, and the comparison of literature values. All the compounds except compound 7 inhibited NO production efficiently with IC50 values of lower than 50 μm. Particularly, compounds 1 and 8 were the most efficient with IC50 values of 25.8 and 27.4 μm, respectively. This is the first report suggesting the potential of S. barbata on the reduction of neuroinflammation.

Keywords

Scutellaria barbata; inflammation; microglial cells; natural products; nitric oxide.

Figures
Products