2. Academic Validation
  3. Insight into the binding of a non-toxic, self-assembling aromatic tripeptide with ct-DNA: Spectroscopic and viscositic studies

Insight into the binding of a non-toxic, self-assembling aromatic tripeptide with ct-DNA: Spectroscopic and viscositic studies

  • Biochem Biophys Rep. 2017 Jul 8;11:112-118. doi: 10.1016/j.bbrep.2017.07.001.
Soumi Biswas 1,  Satyabrata Samui 1,  Arpita Chakraborty 1,  Sagar Biswas 2,  Debapriya De 1,  Utpal Ghosh 1,  Apurba K Das 2,  Jishu Naskar 1
Affiliations

Affiliations

  • 1 Department of Biochemistry & Biophysics, University of Kalyani, Nadia, WB 741235, India.
  • 2 Department of Chemistry, Indian Institute of Technology, Indore, Khandwa Road, Indore 453552, India.
PMID: 28955776 DOI: 10.1016/j.bbrep.2017.07.001
Abstract

The report describes the synthesis, self-association and DNA binding studies of an aromatic tripeptide H-Phe-Phe-Phe-OH (FFF). The peptide backbone adopts β-sheet conformation both in solid and solution. In aqueous solution, FFF self-assembles to form nanostructured aggregates. Interactions of this peptide with calf-thymus DNA (ct-DNA) have been studied using various biophysical techniques including ultraviolet (UV) absorption spectroscopy, fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The value of mean binding constant calculated from UV and fluorescence spectroscopic data is (2.914 ± 0.74) x 103 M-1 which is consistent with an external binding mode. Fluorescence intercalator displacement (FID) assay, iodide quenching study, viscosity measurement and thermal denaturation study of DNA further confirm the groove binding mode of peptide, FFF with ct-DNA. MTT cell survival assay reveals very low cytotoxicity of the peptide toward human lung carcinoma cell line A549.

Keywords

Groove binding; Hyperchromism; Peptide; Self-assembly; ct-DNA.

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