2. Academic Validation
  3. Anti-inflammatory effects of olanexidine gluconate on oral epithelial cells

Anti-inflammatory effects of olanexidine gluconate on oral epithelial cells

  • BMC Oral Health. 2019 Nov 8;19(1):239. doi: 10.1186/s12903-019-0932-0.
Takuya Nii 1,  Hiromichi Yumoto 2,  Katsuhiko Hirota 3 4,  Yoichiro Miyake 3 5
Affiliations

Affiliations

  • 1 Naruto Research Institute, Research and Development Center, Otsuka Pharmaceutical Factory, Inc, Takuya Nii, 115 Kuguhara, Tateiwa, Muya-cho, Naruto, Tokushima, 772-8601, Japan. [email protected].
  • 2 Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • 3 Department of Oral Microbiology, Institute of Biomedical Sciences Tokushima University, Tokushima, Japan.
  • 4 Present Address: Department of Medical Hygiene, Dental Hygiene Course, Kochi Gakuen College, Kochi, Japan.
  • 5 Present Address: Department of Oral Health Sciences, Faculty of Health and Welfare, Tokushima Bunri University, Tokushima, Japan.
PMID: 31703580 DOI: 10.1186/s12903-019-0932-0
Abstract

Background: Periodontitis is a biofilm-induced chronic inflammatory condition of the periodontium. Chemokines produced by the innate and acquired immune responses play a significant role in disease progression. Reducing biofilm formation and inflammatory response caused by chemokines is vital for preventing and treating periodontitis. Previously, we observed that treatment with 0.1% olanexidine gluconate (OLG) inhibited biofilm formation on saliva-coated hydroxyapatite. This study aimed to evaluate the anti-inflammatory effect of OLG on oral epithelial cells.

Methods: We examined if OLG could inhibit the inflammatory responses caused by Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) and heat-killed P. gingivalis in immortalized human oral keratinocytes (RT7).

Results: Treatment of RT7 with non-cytotoxic OLG concentrations significantly inhibited the production of inflammatory chemokines such as interleukin 8 (IL-8), C-C motif ligand 20 (CCL20), and growth-related oncogene protein-α (GRO-α), which are stimulated by P. gingivalis LPS in a concentration-dependent manner. Moreover, the inhibitory effects were observed regardless of the treatment time with P. gingivalis LPS (6, 12, or 24 h). OLG also significantly inhibited chemokine production stimulated by heat-killed P. gingivalis.

Conclusions: The findings of this study suggest that treatment with OLG inhibits chronic inflammatory reactions in oral mucosal cells, such as periodontitis, caused by oral bacteria.

Keywords

Keratinocytes; Olanexidine gluconate; Porphyromonas gingivalis lipopolysaccharide.

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