2. Academic Validation
  3. Inhibitory effects of orientin in mast cell-mediated allergic inflammation

Inhibitory effects of orientin in mast cell-mediated allergic inflammation

  • Pharmacol Rep. 2020 Aug;72(4):1002-1010. doi: 10.1007/s43440-019-00048-3.
Hima Dhakal 1,  Soyoung Lee 2,  Jin Kyeong Choi 3,  Taeg Kyu Kwon 4,  Dongwoo Khang 5,  Sang-Hyun Kim 6
Affiliations

Affiliations

  • 1 Cell and Matrix Research Institute, Department of Pharmacology, School of Medicine, Kyungpook National University, 680, Gukchaebosang-ro, Jung-gu, Daegu, 41944, Republic of Korea.
  • 2 Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea.
  • 3 Molecular Immunology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
  • 4 Department of Immunology, School of Medicine, Keimyung University, Daegu, Republic of Korea.
  • 5 Department of Physiology, School of Medicine, Gachon University, 191, Hambangmoe-ro, Yeonsu-gu, Incheon, Republic of Korea. [email protected].
  • 6 Cell and Matrix Research Institute, Department of Pharmacology, School of Medicine, Kyungpook National University, 680, Gukchaebosang-ro, Jung-gu, Daegu, 41944, Republic of Korea. [email protected].
PMID: 32048267 DOI: 10.1007/s43440-019-00048-3
Abstract

Background: Mast cells are immune effector cells mediating allergic inflammation by the secretion of inflammatory mediators such as histamine and pro-inflammatory cytokines. Orientin is a naturally occurring bioactive flavonoid that possesses diverse biological properties, including anti-inflammation, anti-oxidative, anti-tumor, and cardio protection. The objective of this study was to rule out the effectiveness of orientin in mast cell-mediated allergic inflammation.

Methods: In this study, in vitro effects of orientin were evaluated in RBL-2H3, mouse bone marrow-derived mast cells, rat peritoneal mast cells, and in vivo effects were evaluated by inducing passive cutaneous anaphylaxis (PCA) in Imprinting Control Region (ICR) mice.

Results: Findings show that orientin suppressed the immunoglobulin E (IgE)-mediated mast cell degranulation by reducing intracellular calcium level in a concentration-dependent manner. Orientin suppressed the secretion of pro-inflammatory cytokines in mast cells. This inhibitory effects of orientin was through inhibition of FcεRI-mediated signaling proteins. In addition, oral administration of orientin suppressed the IgE-mediated PCA reactions in a dose-dependent manner, which was evidenced by reduced Evan's blue pigmentation and ear swelling.

Conclusions: Based on these findings, we suggest that orientin might have potential to alleviate allergic reaction and mast cell-mediated allergic disease.

Keywords

Allergic inflammation; Histamine; Mast cell; Orientin; Passive cutaneous anaphylaxis.

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