2. Academic Validation
  3. Fast off-line FPSE-HPLC-PDA determination of six NSAIDs in saliva samples

Fast off-line FPSE-HPLC-PDA determination of six NSAIDs in saliva samples

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2020 May 1;1144:122082. doi: 10.1016/j.jchromb.2020.122082.
A Tartaglia 1,  A Kabir 2,  F D'Ambrosio 1,  P Ramundo 1,  S Ulusoy 3,  H I Ulusoy 4,  G M Merone 5,  F Savini 6,  C D'Ovidio 7,  U De Grazia 8,  K G Furton 9,  M Locatelli 10
Affiliations

Affiliations

  • 1 University of Chieti-Pescara "G. d'Annunzio", Department of Pharmacy, Via dei Vestini 31, Chieti 66100, Italy.
  • 2 International Forensic Research Institute, Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA. Electronic address: [email protected].
  • 3 Department of Pharmacy, Vocational School of Health Service, Cumhuriyet University, Sivas 58140, Turkey.
  • 4 Faculty of Pharmacy, Cumhuriyet University, Department of Analytical Chemistry, Sivas 58140, Turkey.
  • 5 University of Chieti-Pescara "G. d'Annunzio", Department of Neuroscience, Imaging and Clinical Sciences, Via dei Vestini 31, 66100 Chieti, Italy.
  • 6 Hospital "Santo Spirito", Pharmatoxicology Laboratory, Via Fonte Romana 8, Pescara 65124, Italy.
  • 7 University of Chieti-Pescara "G. d'Annunzio", Department of Medicine and Aging Sciences, Section of Legal Medicine, Via dei Vestini 31, Chieti 66100, Italy.
  • 8 Laboratory of Neurological Biochemistry and Neuropharmacology, Fondazione IRCCS Istituto Neurologico Carlo Besta, Via Celoria 11, Milan 20133, Italy.
  • 9 International Forensic Research Institute, Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA.
  • 10 University of Chieti-Pescara "G. d'Annunzio", Department of Pharmacy, Via dei Vestini 31, Chieti 66100, Italy. Electronic address: [email protected].
PMID: 32278291 DOI: 10.1016/j.jchromb.2020.122082
Abstract

A fast off-line FPSE-HPLC-PDA method has been reported that allows simultaneous clean up and determination of six non-steroidal anti-inflammatory drugs (NSAIDs) in saliva samples from healthy volunteers. Particularly, furprofen, indoprofen, ketoprofen, fenbufen, flurbiprofen, and ibuprofen were chromatographically resolved. Benzyl paraben was chosen as the internal standard (BzPB, IS). These target compounds were successfully extracted from human saliva using fabric phase sorptive extraction (FPSE) and then analysed in the liquid chromatographic system by means of a short analytical column (Symmetry C18, 75 × 4.6 mm, 3.5 µm) using acetonitrile (AcN) and phosphate buffer (PBS, 30 mM; pH = 2.5) as the mobile phases. The method, validated through the calculation of all analytical parameters in accordance of International Guidelines, was applied to real saliva sample analysis collected from informed volunteers. The proposed approach that included the use of sol-gel polytetrahydrofuran (sol-gel PTHF) sorbent immobilized on cellulose support and C18 stationary phase used in HPLC, showed high potential as a fast tool for future clinical and forensic applications. The herein reported results encourage potential future application of FPSE in the forensic field. Furthermore, the FPSE membrane was tested in dried saliva spot mode (DSS) in order to check its potential use as a sampling device, also for forensic applications.

Keywords

Fabric phase sorptive extraction; HPLC-PDA; NSAIDs; Saliva samples; Validation.

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