2. Academic Validation
  3. BCAS2, a protein enriched in advanced prostate cancer, interacts with NBS1 to enhance DNA double-strand break repair

BCAS2, a protein enriched in advanced prostate cancer, interacts with NBS1 to enhance DNA double-strand break repair

  • Br J Cancer. 2020 Dec;123(12):1796-1807. doi: 10.1038/s41416-020-01086-y.
Li-Po Wang 1,  Tzu-Yu Chen 2,  Chun-Kai Kang 2,  Hsiang-Po Huang  # 3,  Show-Li Chen  # 4
Affiliations

Affiliations

  • 1 Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2 Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 3 Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan. [email protected].
  • 4 Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan. [email protected].
  • # Contributed equally.
PMID: 32963349 DOI: 10.1038/s41416-020-01086-y
Abstract

Background: Breast Cancer amplified sequence 2 (BCAS2) plays crucial roles in pre-mRNA splicing and Androgen Receptor transcription. Previous studies suggested that BCAS2 is involved in double-strand breaks (DSB); therefore, we aimed to characterise its mechanism and role in prostate Cancer (PCa).

Methods: Western blotting and immunofluorescence microscopy were used to assay the roles of BCAS2 in the DSBs of PCa cells and Apoptosis in Drosophila, respectively. The effect of BCAS2 dosage on non-homologous end joining (NHEJ) and homologous recombination (HR) were assayed by precise end-joining assay and flow cytometry, respectively. Glutathione-S-transferase pulldown and co-immunoprecipitation assays were used to determine whether and how BCAS2 interacts with NBS1. The expression of BCAS2 and other proteins in human PCa was determined by immunohistochemistry.

Results: BCAS2 helped repair radiation-induced DSBs efficiently in both human PCa cells and Drosophila. BCAS2 enhanced both NHEJ and HR, possibly by interacting with NBS1, which involved the BCAS2 N-terminus as well as both the NBS1 N- and C-termini. The overexpression of BCAS2 was significantly associated with higher Gleason and pathology grades and shorter survival in patients with PCa.

Conclusion: BCAS2 promotes two DSB repair pathways by interacting with NBS1, and it may affect PCa progression.

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