2. Academic Validation
  3. Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

  • Sci Rep. 2021 Jan 18;11(1):1690. doi: 10.1038/s41598-020-79702-3.
Jessie L Webber # 1 2,  Rashin Namivandi-Zangeneh # 3,  Sławomir Drozdek 1 4,  Kazimiera A Wilk 4,  Cyrille Boyer 3,  Edgar H H Wong 3,  Bronwyn H Bradshaw-Hajek 2,  Marta Krasowska 5 6,  David A Beattie 7 8
Affiliations

Affiliations

  • 1 Future Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia.
  • 2 UniSA STEM, University of South Australia, Mawson Lakes, SA, 5095, Australia.
  • 3 Centre for Advanced Macromolecular Design and Australian Centre for NanoMedicine, School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
  • 4 Department of Engineering and Technology of Chemical Processes, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
  • 5 Future Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia. [email protected].
  • 6 UniSA STEM, University of South Australia, Mawson Lakes, SA, 5095, Australia. [email protected].
  • 7 Future Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia. [email protected].
  • 8 UniSA STEM, University of South Australia, Mawson Lakes, SA, 5095, Australia. [email protected].
  • # Contributed equally.
PMID: 33462270 DOI: 10.1038/s41598-020-79702-3
Abstract

An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural Polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm-2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.

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