Nisin Z
Based on 1 Customer Validation
Nisin Z is an antimicrobial and anti-inflammatory peptide. Nisin Z is effective against Gram-positive bacteria and fungi, such as C. albicans.
For research use only. We do not sell to patients.
- CAS No.: 137061-46-2
- Formula: C141H229N41O38S7
- Molecular Weight:3331.05
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Nisin Z (10 μg/mL, 24 h) increases gingival fibroblasts detachment and differentiation[2].
Nisin Z (25-75 μg/mL, 24 h) decreases C. albicans adhesion to the gingival cells[2].
Nisin Z (500 and 1000 μg/mL, 24 h) inhibits germ tube formation by C. albicans[3].
Nisin Z (100 μg/mL, 12 h) reduces the production of LPS-induced pro-inflammatory cytokines (i.e., IL-6, TNF-α, IL-1β) in MCF10A cells[4].
Nisin Z (10-100 μg/mL, 12 h) inhibits the activation of the ERK1/2 and p38 MAPK signaling pathway in LPS-induced MCF10A cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C. albicans
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Concentration:100, 500, 1000 μg/mL
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Incubation Time:24, 48, and 72 h
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Result:Reduced C. albicans growth.
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Cell Line:LPS-induced MCF10A cells
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Concentration:10-100 μg/mL
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Incubation Time:12 h
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Result:Inhibited the phosphorylation of p38 and and ERK1/2.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mastitis mouse model[4]
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Dosage:1, 5, and 10 mg/kg
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Administration:i.p., one hour before treatment of LPS.
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Result:Reduced inflammatory cell infiltration.
Showed a reduction in inflammatory cytokine levels.
Chemical Information
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CAS No. 137061-46-2
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Appearance Solid
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Molecular Weight 3331.05
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Formula C141H229N41O38S7
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Color Off-white to light brown
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Sequence
Ile-Dhb-Ala-Ile-Dha-Leu-Ala-Abu-Pro-Gly-Ala-Lys-Abu-Gly-Ala-Leu-Met-Gly-Ala-Asn-Met-Lys-Abu-Ala-Abu-Ala-Asn-Ala-Ser-Ile-His-Val-Dha-Lys (Sulfide bridge: Ala3-Ala7, Abu8-Ala11, Abu13-Ala19, Abu23-Ala26, Abu25-Ala28)
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Sequence Shortening
I-Dhb-AI-Dha-LA-Abu-PGAK-Abu-GALMGANMK-Abu-A-Abu-ANASIHV-Dha-K (Sulfide bridge: Ala3-Ala7, Abu8-Ala11, Abu13-Ala19, Abu23-Ala26, Aba25-Ala28)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
0.1 M HCl : 20 mg/mL (6.00 mM; adjust pH to 1 with H2O)
H2O : 10 mg/mL (3.00 mM; Need ultrasonic)
DMSO : 2 mg/mL (0.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% Saline
Solubility: ≥ 0.2 mg/mL (0.06 mM); Clear solution
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.14 mg/mL (0.04 mM); Clear solution
This protocol yields a clear solution of ≥ 0.14 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (1.4 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (306 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Webber JL, et al. Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers. Sci Rep. 2021 Jan 18;11(1):1690. [Content Brief]
[2]. Akerey B, et al. In vitro efficacy of nisin Z against Candida albicans adhesion and transition following contact with normal human gingival cells. J Appl Microbiol. 2009 Oct;107(4):1298-307. [Content Brief]
[3]. Le Lay C, et al. Nisin Z inhibits the growth of Candida albicans and its transition from blastospore to hyphal form. J Appl Microbiol. 2008 Nov;105(5):1630-9. [Content Brief]
[4]. Huang F, et al. Nisin Z attenuates lipopolysaccharide-induced mastitis by inhibiting the ERK1/2 and p38 mitogen-activated protein kinase signaling pathways. J Dairy Sci. 2022 Apr;105(4):3530-3543. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / 0.1 M HCl | 1 mM | 0.3002 mL | 1.5010 mL | 3.0021 mL | 7.5051 mL |
| 0.1 M HCl | 5 mM | 0.0600 mL | 0.3002 mL | 0.6004 mL | 1.5010 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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