Nisin
Based on 3 publication(s) in Google Scholar
Nisin is a bacteriocin produced by a group of Gram-positive bacteria that belongs to Lactococcus and Streptococcus species. Nisin has antibacterial and anti-inflammatory activity.
For research use only. We do not sell to patients.
- CAS No.: 1414-45-5
- Formula: C143H230N42O37S7
- Molecular Weight:3354.07
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Nisin
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Customer Validation & Images
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Cell Proliferation/Viability Assay
All Antibiotic Isoforms
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Biological Activity
Nisin (100-800 μg/mL, 36 h) inhibits HNSCC cell ballooning[1].
Nisin (500-7000 IU/mL, 24 h) induces free-living amoeba acanthamoeba cell cycle arrest[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A. castellanii trophozoites
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Concentration:500, 1000, 2000, 3000, 4000, 6000, 7000 IU/mL
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Incubation Time:24, 72 h
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Result:Inhibited amebic growth in a concentration-dependent manner at 24 h.
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Cell Line:A. castellanii trophozoites
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Concentration:7000 IU/mL
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Incubation Time:24 h
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Result:Increased S phase to 13.3% and the percentage of G1 to 11.1%.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat uterine endometritis model[3]
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Dosage:25 mg/kg
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Administration:i.p. for 7 days
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Result:Decreased the endometritis-induced increase in uterine weight, restored endometrial architecture.
Improved levels of B7-2, IFN-γ, IL-2 and IL-8.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Ile-Dhb-Ala-Ile-Dha-Leu-Ala-Abu-Pro-Gly-Ala-Lys-Abu-Gly-Ala-Leu-Met-Gly-Ala-Asn-Met-Lys-Abu-Ala-Aba-Ala-His-Ala-Ser-Ile-His-Val-Dha-Lys (Sulfide bridge: Ala3-Ala7, Abu8-Ala11, Abu13-Ala19, Abu23-Ala26, Aba25-Ala28)
I-Dhb-AI-Dha-LA-Abu-PGAK-Abu-GALMGANMK-Abu-A-Aba-AHASIHV-Dha-K (Sulfide bridge: Ala3-Ala7, Abu8-Ala11, Abu13-Ala19, Abu23-Ala26, Aba25-Ala28)
Chemical Information
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CAS No. 1414-45-5
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Appearance Solid
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Molecular Weight 3354.07
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Formula C143H230N42O37S7
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Color Off-white to light brown
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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J Hazard Mater
Development of transcriptional factor-based whole-cell biosensors to monitor and degrade antibiotics using mutant cells obtained via adaptive laboratory evolution. [Abstract]2024 Jul 15:473:134536. PMID: 38759406
Nisin purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2024 Jul 15:473:134536. [Abstract]
Nisin (6-60 nM) greatly diminished growth and biosensor performance of L. lactis NZ9000.
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Vaccine
Assessment of recombinant Lactobacillus vector-based expressing IFITM3 protein anti-PRV infection in mice. [Abstract]2025 Apr 16:54:127093. PMID: 40245770 -
Solvent & Solubility
H2O : 6.67 mg/mL (1.99 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (301 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Shin JM, et al. Biomedical applications of nisin. J Appl Microbiol. 2016 Jun;120(6):1449-65. [Content Brief]
[2]. de Carvalho Clímaco M, et al. Nisin Induces Cell-Cycle Arrest in Free-Living Amoebae Acanthamoeba castellanii. Acta Parasitol. 2022 Mar;67(1):511-517. [Content Brief]
[3]. Jia Z, et al. Nisin reduces uterine inflammation in rats by modulating concentrations of pro- and anti-inflammatory cytokines. Am J Reprod Immunol. 2019 May;81(5):e13096. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.2981 mL | 1.4907 mL | 2.9815 mL | 7.4536 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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