2. Academic Validation
  3. Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans

Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans

  • Sci Adv. 2021 Feb 3;7(6):eaaz9857. doi: 10.1126/sciadv.aaz9857.
Pavlo V Khodakivskyi 1 2,  Christian L Lauber 2,  Aleksey Yevtodiyenko 2,  Arkadiy A Bazhin 2,  Stephen Bruce 3,  Tamar Ringel-Kulka 4,  Yehuda Ringel 5 6 7,  Bertrand Bétrisey 8,  Joana Torres 9 10,  Jianzhong Hu 11,  Chieh Jason Chou 12,  Elena A Goun 13 2
Affiliations

Affiliations

  • 1 Department of Chemistry, University of Missouri, Columbia, MO 65211, USA.
  • 2 Institute of Chemical Sciences and Engineering (ISIC), Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
  • 3 Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland.
  • 4 UNC Gillings School of Global Public Health, University of North Carolina at Chapel Hill , Chapel Hill, NC 27599, USA.
  • 5 Division of Gastroenterology and Hepatology, Meir Medical Center, affiliated with Tel Aviv University, Kfar-Saba, Israel.
  • 6 Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
  • 7 Biomica Ltd. 13 Gad Feinstein St. POB 4173, Rehovot 7414002, Israel.
  • 8 Cellular Metabolism, Department of Cell Biology, Nestlé Institute of Health Sciences, Nestlé Research, 1000 Lausanne, Switzerland.
  • 9 Gastroenterology Division, Hospital Beatriz Ângelo, Loures, Portugal.
  • 10 The Dr. Henry D. Janowitz Division of Gastroenterology, Icahn School of Medicine at Mount Sinai, New York City, NY 10029, USA.
  • 11 Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • 12 Department of Gastro-Intestinal Health and Microbiome, Nestlé Institute of Health Sciences, Nestlé Research, 1000 Lausanne, Switzerland.
  • 13 Department of Chemistry, University of Missouri, Columbia, MO 65211, USA. [email protected].
PMID: 33536224 DOI: 10.1126/sciadv.aaz9857
Abstract

The microbiome-produced Enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used for quick and cost-effective quantification of BSH activity across a broad range of biological settings including pure enzymes and bacteria, intact fecal slurries, and noninvasive imaging in live Animals, as well as for the assessment of BSH activity in the entire gastrointestinal tract of mice and humans. Using this assay, we showed that certain types of prebiotics are capable of increasing BSH activity of the gut microbiota in vivo and successfully demonstrated potential application of this assay as a noninvasive diagnostic test to predict the clinical status of inflammatory bowel disease (IBD) patients.

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