2. Academic Validation
  3. PCTR1 Enhances Repair and Bacterial Clearance in Skin Wounds

PCTR1 Enhances Repair and Bacterial Clearance in Skin Wounds

  • Am J Pathol. 2021 Jun;191(6):1049-1063. doi: 10.1016/j.ajpath.2021.02.015.
Brian E Sansbury 1,  Xiaofeng Li 1,  Blenda Wong 1,  Colin O Riley 1,  Ashley E Shay 1,  Robert Nshimiyimana 2,  Nicos A Petasis 3,  Charles N Serhan 1,  Matthew Spite 4
Affiliations

Affiliations

  • 1 Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
  • 2 Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts; Department of Chemistry and Loker Hydrocarbon Research Institute, University of Southern California, Los Angeles, California.
  • 3 Department of Chemistry and Loker Hydrocarbon Research Institute, University of Southern California, Los Angeles, California.
  • 4 Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts. Electronic address: [email protected].
PMID: 33689792 DOI: 10.1016/j.ajpath.2021.02.015
Abstract

Tissue injury elicits an inflammatory response that facilitates host defense. Resolution of inflammation promotes the transition to tissue repair and is governed, in part, by specialized pro-resolving mediators (SPM). The complete structures of a novel series of cysteinyl-SPM (cys-SPM) were recently elucidated, and proved to stimulate tissue regeneration in planaria and resolve acute inflammation in mice. Their functions in mammalian tissue repair are of interest. Here, nine structurally distinct cys-SPM were screened and PCTR1 uniquely enhanced human keratinocyte migration with efficacy similar to epidermal growth factor. In skin wounds of mice, PCTR1 accelerated closure. Wound Infection increased PCTR1 that coincided with decreased Bacterial burden. Addition of PCTR1 reduced wound bacteria levels and decreased inflammatory monocytes/macrophages, which was coupled with increased expression of genes involved in host defense and tissue repair. These results suggest that PCTR1 is a novel regulator of host defense and tissue repair, which could inform new approaches for therapeutic management of delayed tissue repair and Infection.

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