2. Academic Validation
  3. Anti-Inflammatory Effects of an Extract from Pseudomonas aeruginosa and Its Purified Product 1-Hydroxyphenazine on RAW264.7 Cells

Anti-Inflammatory Effects of an Extract from Pseudomonas aeruginosa and Its Purified Product 1-Hydroxyphenazine on RAW264.7 Cells

  • Curr Microbiol. 2021 Jul;78(7):2762-2773. doi: 10.1007/s00284-021-02544-3.
Jun Xiao 1 2,  Aye Aye Thwe 1 2,  Tingting Liu 1 2,  Dafei Gong 1 2,  Wanhua Lin 1 3,  Changhua Shang 4 5,  ZuJun Lu 6 7
Affiliations

Affiliations

  • 1 College of Life Science, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
  • 2 Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
  • 3 Key Laboratory of Stem Cell and Biopharmaceutical Technology, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
  • 4 College of Life Science, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China. [email protected].
  • 5 Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China. [email protected].
  • 6 College of Life Science, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China. [email protected].
  • 7 Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China. [email protected].
PMID: 34043026 DOI: 10.1007/s00284-021-02544-3
Abstract

The purpose of this study was to discuss the effects of an extract from the culture medium of Pseudomonas aeruginosa (P. aeruginosa) 2016NX1 (chloroform extract of P. aeruginosa, CEPA) and its purified product 1-hydroxyphenazine on RAW264.7 cell inflammation. Cell viability was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method. TNF-α production was determined by an ELISA method. The effects of CEPA and its purified product 1-hydroxyphenazine on cell morphology were investigated using an inverted microscope. Quantitative Real-Time PCR was performed to determine mRNA expression levels. CEPA and 1-hydroxyphenazine had no obvious toxicity to cells when their concentrations were no more than 20 μg ml-1 and 5 μg ml-1, respectively. Both CEPA and 1-hydroxyphenazine suppressed the secretion of TNF-α and significantly reduced the mRNA expression levels of TNF-α, IL-1β, and IL-6. Both CEPA and 1-hydroxyphenazine inhibited M1 cell polarization after lipopolysaccharide (LPS) stimulation. The results in this article lay a good foundation for the biopharmaceutical applications of CEPA and 1-hydroxyphenazine in the future. CEPA and 1-hydroxyphenazine had certain anti-inflammatory activity, and inhibited LPS-induced RAW264.7 cell inflammation. Our findings suggest that CEPA and 1-hydroxyphenazine are potential chemicals with anti-inflammatory activity.

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