1-Hydroxyphenazine
Based on 1 Customer Validation
1-Hydroxyphenazine (Hemipyocyanine; 1-Phenazinol; Hemi-pyocyanin) is an inhibitor for α-Amylase with an IC50 of 3.1 μg/mL. 1-Hydroxyphenazine exhibits anticancer and anti-inflammatory activity against cells A549, 1321N1 and RAW264.7, antifungal and antibacterial activity against strains Candida albicans, Aspergillus fumigatus, Escherichia coli and Xanthomonas campestris.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 528-71-2
- Formula: C12H8N2O
- Molecular Weight:196.20
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
1-Hydroxyphenazine (0.1-100 μM) inhibits proliferation against brain astrocytoma 1321N1 cells through formation of acidic vesicular organelle, which is a hallmark of cell autophagy, exhibits potential toxicity in central nervous system (CNS)[2].
Hydroxyphenazine (0-10 μg/mL) inhibits secretion of TNF-α and M1 cell polarization, inhibits LPS induced inflammation in RAW264.7 cells without significant toxicity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7
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Concentration:0-10 μg/mL
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Incubation Time:24 h
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Result:Inhibited proliferation of RAW264.7 in a dose-dependent maner.
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Cell Line:RAW264.7
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Concentration:0-10 μg/mL
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Incubation Time:24 h
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Result:Decreased levels of TNF-α, IL-1β and IL-6 mRNA expressions.
Chemical Information
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CAS No. 528-71-2
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Appearance Solid
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Molecular Weight 196.20
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Formula C12H8N2O
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Color Yellow to orange
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SMILES
OC1=CC=CC2=NC3=CC=CC=C3N=C21
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Synonyms
Hemipyocyanine; 1-Phenazinol; Hemi-pyocyanin
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 100 mg/mL (509.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Nguyen TH, et al., Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards. Mar Drugs. 2022 Apr 23;20(5):283. [Content Brief]
[2]. McFarland AJ, et al., Inhibition of autophagy by 3-methyladenine protects 1321N1 astrocytoma cells against pyocyanin- and 1-hydroxyphenazine-induced toxicity. Arch Toxicol. 2012 Feb;86(2):275-84 [Content Brief]
[3]. Xiao J, et al., Anti-Inflammatory Effects of an Extract from Pseudomonas aeruginosa and Its Purified Product 1-Hydroxyphenazine on RAW264.7 Cells. Curr Microbiol. 2021 Jul;78(7):2762-2773. [Content Brief]
[4]. Prabhu MS, et al., Purification and molecular and biological characterisation of the 1-hydroxyphenazine, produced by an environmental strain of Pseudomonas aeruginosa. World J Microbiol Biotechnol. 2014 Dec;30(12):3091-9. [Content Brief]
[5]. Kerr JR, et al., Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth. J Clin Pathol. 1999 May;52(5):385-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.0968 mL | 25.4842 mL | 50.9684 mL | 127.4210 mL |
| 5 mM | 1.0194 mL | 5.0968 mL | 10.1937 mL | 25.4842 mL | |
| 10 mM | 0.5097 mL | 2.5484 mL | 5.0968 mL | 12.7421 mL | |
| 15 mM | 0.3398 mL | 1.6989 mL | 3.3979 mL | 8.4947 mL | |
| 20 mM | 0.2548 mL | 1.2742 mL | 2.5484 mL | 6.3711 mL | |
| 25 mM | 0.2039 mL | 1.0194 mL | 2.0387 mL | 5.0968 mL | |
| 30 mM | 0.1699 mL | 0.8495 mL | 1.6989 mL | 4.2474 mL | |
| 40 mM | 0.1274 mL | 0.6371 mL | 1.2742 mL | 3.1855 mL | |
| 50 mM | 0.1019 mL | 0.5097 mL | 1.0194 mL | 2.5484 mL | |
| 60 mM | 0.0849 mL | 0.4247 mL | 0.8495 mL | 2.1237 mL | |
| 80 mM | 0.0637 mL | 0.3186 mL | 0.6371 mL | 1.5928 mL | |
| 100 mM | 0.0510 mL | 0.2548 mL | 0.5097 mL | 1.2742 mL |
1-Hydroxyphenazine Related Classifications
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