2. Academic Validation
  3. A comprehensive assessment of the biocompatibility of Magnetospirillum gryphiswaldense MSR-1 bacterial magnetosomes in vitro and in vivo

A comprehensive assessment of the biocompatibility of Magnetospirillum gryphiswaldense MSR-1 bacterial magnetosomes in vitro and in vivo

  • Toxicology. 2021 Oct;462:152949. doi: 10.1016/j.tox.2021.152949.
Xiaohui Nan 1,  Yan Teng 2,  Jiesheng Tian 3,  Zhiyuan Hu 4,  Qiaojun Fang 5
Affiliations

Affiliations

  • 1 Laboratory of Theoretical and Computational Nanoscience, CAS Key Laboratory of Nanophotonic Materials and Devices, CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Ambient Particles Health Effects and Prevention Techniques, National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • 2 Wedocare Inc, Beijing, 100190, China.
  • 3 State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
  • 4 Laboratory of Theoretical and Computational Nanoscience, CAS Key Laboratory of Nanophotonic Materials and Devices, CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Ambient Particles Health Effects and Prevention Techniques, National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing, 100190, China. Electronic address: [email protected].
  • 5 Laboratory of Theoretical and Computational Nanoscience, CAS Key Laboratory of Nanophotonic Materials and Devices, CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Ambient Particles Health Effects and Prevention Techniques, National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing, 100190, China. Electronic address: [email protected].
PMID: 34534559 DOI: 10.1016/j.tox.2021.152949
Abstract

Bacterial magnetosomes (BMs) are iron oxide nanoparticles synthesized naturally by magnetotactic bacteria, made up of nano-sized inorganic crystals enclosed by a lipid bilayer membrane. Due to several superior characteristics, such as the narrow size distribution, uniform morphology, high purity and crystallinity, single magnetic domain as well as easy surface modification, increasing biomedical and biotechnological applications of BMs have been developed. The attracted wide attentions raise the urge for the evaluation of safety and toxicity. In this work, we performed a rather comprehensive and systematic assessment of in vitro and in vivo toxicity of BMs from MSR-1, including the cytotoxicity, mice bodyweights, blood test, organ coefficients, inflammation, and hemocompatibility study. We found that BMs have good biocompatibility except for influences on the immune response as demonstrated by enhanced activation of the Complement System and inhibition of lymphocyte proliferation when used with an excessive concentration. BMs induced the production of Reactive Oxygen Species (ROS) in macrophages at a dose-dependent manner but did not cause cell membrane damage and cell cycle arrest until the concentration is approximately 40 times the clinical dosage. We anticipate our work will guide modifications of BMs and expand their future applications.

Keywords

Bacterial magnetosomes (BMs); Biocompatibility; Cytotoxicity; Hemocompatibility; Inflammation; Reactive oxygen species (ROS).

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