1. Fluorescent Dye
  2. Cell staining analysis
    Biology Analysis
  3. Apoptosis
    Cell Cycle Assay
    Cell Viability
    Cytotoxicity Assay
    Bacterial Localization
  4. Thiazolyl Blue

Thiazolyl Blue  (Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

Cat. No.: HY-15924 Purity: 99.84%
COA Handling Instructions

Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

For research use only. We do not sell to patients.

Thiazolyl Blue Chemical Structure

Thiazolyl Blue Chemical Structure

CAS No. : 298-93-1

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 55 Get quote
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 Get quote
Solid
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 130 In-stock
Estimated Time of Arrival: December 31
10 g   Get quote  
50 g   Get quote  

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Customer Review

Based on 77 publication(s) in Google Scholar

Top Publications Citing Use of Products

77 Publications Citing Use of MCE Thiazolyl Blue

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

In Vitro

Thiazolyl Blue (MTT) is used in conjunction with rhodamine B to measure mitochondrial membrane potential. Thiazolyl Blue-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of Thiazolyl Blue is strong. Thiazolyl Blue is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

414.32

Formula

C18H16BrN5S

CAS No.
SMILES

CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (60.34 mM; ultrasonic and warming and heat to 60°C)

H2O : 0.88 mg/mL (2.12 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4136 mL 12.0680 mL 24.1359 mL
5 mM 0.4827 mL 2.4136 mL 4.8272 mL
10 mM 0.2414 mL 1.2068 mL 2.4136 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 6.67 mg/mL (16.10 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.02 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.02 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.84%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Thiazolyl Blue
Cat. No.:
HY-15924
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