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  2. Thiazolyl Blue

Thiazolyl Blue (Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

Cat. No.: HY-15924 Purity: 99.59%
Handling Instructions

Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

For research use only. We do not sell to patients.

Thiazolyl Blue Chemical Structure

Thiazolyl Blue Chemical Structure

CAS No. : 298-93-1

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
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5 g USD 130 In-stock
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Customer Review

Based on 15 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

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Description

Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

In Vitro

Thiazolyl Blue (MTT) is used in conjunction with rhodamine B to measure mitochondrial membrane potential. Thiazolyl Blue-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of Thiazolyl Blue is strong. Thiazolyl Blue is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].

Molecular Weight

414.32

Formula

C₁₈H₁₆BrN₅S

CAS No.

298-93-1

SMILES

CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 32 mg/mL (77.23 mM)

H2O : 0.88 mg/mL (2.12 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4136 mL 12.0680 mL 24.1359 mL
5 mM 0.4827 mL 2.4136 mL 4.8272 mL
10 mM 0.2414 mL 1.2068 mL 2.4136 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 99.59%

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Keywords:

Thiazolyl BlueMTTThiazolyl Blue TetrazoliumMethylthiazolyldiphenyl-tetrazoliumOthersInhibitorinhibitorinhibit

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Product name:
Thiazolyl Blue
Cat. No.:
HY-15924
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