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Thiazolyl Blue  (Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

Cat. No.: HY-15924 Purity: 99.84%
COA Handling Instructions

Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.

For research use only. We do not sell to patients.

Thiazolyl Blue Chemical Structure

Thiazolyl Blue Chemical Structure

CAS No. : 298-93-1

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Based on 95 publication(s) in Google Scholar

Top Publications Citing Use of Products

95 Publications Citing Use of MCE Thiazolyl Blue

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Description

Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis[1].

In Vitro

1.Preparation of MTT working solution
Dissolve MTT by PBS to obtain 5 mg/mL of MTT.
2.Cell proliferation detection ( 96-well plates)
2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.
2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h.
2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.
2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely
2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

414.32

Formula

C18H16BrN5S

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (60.34 mM; ultrasonic and warming and heat to 60°C)

H2O : 0.88 mg/mL (2.12 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4136 mL 12.0680 mL 24.1359 mL
5 mM 0.4827 mL 2.4136 mL 4.8272 mL
10 mM 0.2414 mL 1.2068 mL 2.4136 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 6.67 mg/mL (16.10 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.02 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.02 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.84%

References
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Thiazolyl Blue Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Thiazolyl Blue
Cat. No.:
HY-15924
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