1. Biochemical Assay Reagents

MTT (Synonyms: Thiazolyl Blue; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

Cat. No.: HY-15924 Purity: 99.59%
Handling Instructions

MTT, a yellow tetrazole, is reduced to purple formazan in living cells, and used for assessing cell metabolic activity.

For research use only. We do not sell to patients.

MTT Chemical Structure

MTT Chemical Structure

CAS No. : 298-93-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 106 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
1 g USD 96 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 g USD 384 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

    MTT purchased from MCE. Usage Cited in: RSC Adv. 11th May 2018.

    The NH2 cells expressing Tat. All tested cells are treated with SAHA for 24 h. The expression of GFP for tested J-Lat A2 and 2D10 cells is measured by FACS. Whole cell extracts for tested NH1 and NH2 cells are prepared and examined for the luciferase activities.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    MTT, a yellow tetrazole, is reduced to purple formazan in living cells, and used for assessing cell metabolic activity.

    In Vitro

    MTT is used in conjunction with rhodamine B to measure mitochondrial membrane potential. MTT-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of MTT is strong. MTT is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].

    Clinical Trial
    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 2.4136 mL 12.0680 mL 24.1359 mL
    5 mM 0.4827 mL 2.4136 mL 4.8272 mL
    10 mM 0.2414 mL 1.2068 mL 2.4136 mL
    Please refer to the solubility information to select the appropriate solvent.
    References
    Molecular Weight

    414.32

    Formula

    C₁₈H₁₆BrN₅S

    CAS No.

    298-93-1

    SMILES

    CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 32 mg/mL; H2O: 0.88 mg/mL (Need ultrasonic and warming)

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: 99.59%

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    MTT
    Cat. No.:
    HY-15924
    Quantity: