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  4. Thiazolyl Blue

Thiazolyl Blue  (Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

Cat. No.: HY-15924 Purity: 99.84%
COA
SDS
Handling Instructions Technical Support

Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.

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Thiazolyl Blue

Thiazolyl Blue Chemical Structure

CAS No. : 298-93-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
10 g In-stock
50 g   Get quote  

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Customer Review

Based on 182 publication(s) in Google Scholar

Other Forms of Thiazolyl Blue:

Thiazolyl Blue Related Antibodies

Top Publications Citing Use of Products

182 Publications Citing Use of MCE Thiazolyl Blue

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis[1].

In Vitro

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of MTT solution
Dissolve MTT in anhydrous DMSO to obtain a 5 mg/mL solution.
2. Cell proliferation assay (96-well plate)
2.1 Cell inoculation: Prepare single cells and prepare a suspension with 10% FBS culture medium. Inoculate them into a 96-well plate, with 1000-10,000 cells per well, and each well volume is 100 μL.
2.2 Cell culture: At 37°C, with 5% CO2, incubate for 24-72 hours.
2.3 Add 10 μL of MTT to each well, incubate for 4 hours, discard the supernatant, and the cells need to be centrifuged first.
2.4 Add 100 μL of DMSO, shake for 10 minutes to completely dissolve the crystals.
2.5 Use an absorbance plate reader to monitor the increase in absorbance at OD = 562 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Thiazolyl Blue Related Antibodies
Molecular Weight

414.32

Formula

C18H16BrN5S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (60.34 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 0.88 mg/mL (2.12 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4136 mL 12.0680 mL 24.1359 mL
5 mM 0.4827 mL 2.4136 mL 4.8272 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.84%

SDS (419 KB)

COA (256 KB) HNMR (222 KB) LCMS (125 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.4136 mL 12.0680 mL 24.1359 mL 60.3398 mL
DMSO 5 mM 0.4827 mL 2.4136 mL 4.8272 mL 12.0680 mL
10 mM 0.2414 mL 1.2068 mL 2.4136 mL 6.0340 mL
15 mM 0.1609 mL 0.8045 mL 1.6091 mL 4.0227 mL
20 mM 0.1207 mL 0.6034 mL 1.2068 mL 3.0170 mL
25 mM 0.0965 mL 0.4827 mL 0.9654 mL 2.4136 mL
30 mM 0.0805 mL 0.4023 mL 0.8045 mL 2.0113 mL
40 mM 0.0603 mL 0.3017 mL 0.6034 mL 1.5085 mL
50 mM 0.0483 mL 0.2414 mL 0.4827 mL 1.2068 mL
60 mM 0.0402 mL 0.2011 mL 0.4023 mL 1.0057 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Thiazolyl Blue Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thiazolyl Blue298-93-1MTTThiazolyl Blue TetrazoliumMethylthiazolyldiphenyl-tetrazoliumFluorescent DyeInhibitorinhibitorinhibit

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