1. Biochemical Assay Reagents

MTT (Synonyms: Thiazolyl Blue; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

Cat. No.: HY-15924 Purity: 99.59%
Data Sheet SDS Handling Instructions

MTT, a yellow tetrazole, is reduced to purple formazan in living cells, and used for assessing cell metabolic activity.

For research use only. We do not sell to patients.
MTT Chemical Structure

MTT Chemical Structure

CAS No. : 298-93-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $88 In-stock
1 g $80 In-stock
5 g $320 In-stock
10 g   Get quote  
50 g   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

MTT, a yellow tetrazole, is reduced to purple formazan in living cells, and used for assessing cell metabolic activity.

In Vitro

MTT is used in conjunction with rhodamine B to measure mitochondrial membrane potential. MTT-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of MTT is strong. MTT is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.4136 mL 12.0680 mL 24.1359 mL
5 mM 0.4827 mL 2.4136 mL 4.8272 mL
10 mM 0.2414 mL 1.2068 mL 2.4136 mL
Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

414.32

Formula

C₁₈H₁₆BrN₅S

CAS No.

298-93-1

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 32 mg/mL; H2O: 0.88 mg/mL (Need ultrasonic and warming)

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 99.59%

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MTT
Cat. No.:
HY-15924
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